Actin polymerization-driven molecular movement of mDia1 in living cells
Actin polymerization-driven molecular movement of mDia1 in living cells
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DOI：
10.1126/science.1093923
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发表时间：
2004-03-26
期刊：
影响因子：
56.9
通讯作者：
Watanabe, N
中科院分区：
文献类型：
--
作者：
Higashida, C;Miyoshi, T;Watanabe, N
mDia1是一种Rho效应蛋白，属于Formin蛋白家族，具有保守的串联FH1-FH2单元结构。包括mDia1在内的形成蛋白在与肌动蛋白相互作用时加速肌动蛋白成核。哀叹快速增长的终结。在这里，我们的单分子成像显示mDia1 FH1-FH2在活细胞中快速定向运动数十微米。mDia1 FH1-FH2的运动被肌动蛋白干扰药物阻断，mDia1 FH1-FH2的运动速度似乎与肌动蛋白延伸率相关。在体外，mDia1 FH1-FH2持续与生长的肌动蛋白倒刺端相关。mDia1可能沿着肌动蛋白的生长端前进。福明斯可能是一种独立于运动蛋白的分子运动机制。
mDia1, a Rho effector, belongs to the Formin family of proteins, which shares the conserved tandem FH1-FH2 unit structure. Formins including mDia1 accelerate actin nucleation while interacting with actin. lament fast-growing ends. Here our single-molecule imaging revealed fast directional movement of mDia1 FH1-FH2 for tens of microns in living cells. The movement of mDia1 FH1-FH2 was blocked by actin-perturbing drugs, and the speed of mDia1 FH1-FH2 movement appeared to correlate with actin elongation rates. In vitro, mDia1 FH1-FH2 associated persistently with the growing actin barbed end. mDia1 probably moves processively along the growing end of actin. laments in cells, and Formins may be a molecular motility machinery that is independent from motor proteins.