Mass Spectrometric Analysis of Exhaled Breath for the Identification of Volatile Organic Compound Biomarkers in Esophageal and Gastric Adenocarcinoma
Mass Spectrometric Analysis of Exhaled Breath for the Identification of Volatile Organic Compound Biomarkers in Esophageal and Gastric Adenocarcinoma
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DOI：
10.1097/sla.0000000000001101
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发表时间：
2015-12-01
影响因子：
9
通讯作者：
Hanna, George B.
中科院分区：
文献类型：
--
作者：
Kumar, Sacheen;Huang, Juzheng;Hanna, George B.
目的：本研究评估使用选择离子流管质谱呼出气分析是否可以区分食管和胃腺癌noncancer controls.Background：大多数上消化道癌患者存在晚期疾病，导致长期生存率差。需要新的方法来诊断潜在的可治愈的上消化道malignances.Methods：轮廓-3选择离子流管质谱仪用于分析呼出气样本中的挥发性有机化合物（VOCs）。所有研究参与者在呼吸采样当天接受了上消化道内窥镜检查。接受者操作特征分析和诊断风险预测模型被用来评估识别的VOCs的判别准确性。结果：呼气样本进行了分析，从81例食管癌（N=48）或胃腺癌（N=33）和129个控制，包括巴雷特化生（N=16），良性上消化道疾病（N=62），或正常的上消化道（N=51）。十二种挥发性有机化合物戊酸、己酸、苯酚、甲基苯酚、乙基苯酚、丁醛、戊醛、己醛、庚醛、辛醛、壬醛和癸醛的浓度明显较高（P
Objective:The present study assessed whether exhaled breath analysis using Selected Ion Flow Tube Mass Spectrometry could distinguish esophageal and gastric adenocarcinoma from noncancer controls.Background:The majority of patients with upper gastrointestinal cancer present with advanced disease, resulting in poor long-term survival rates. Novel methods are needed to diagnose potentially curable upper gastrointestinal malignancies.Methods:A Profile-3 Selected Ion Flow Tube Mass Spectrometry instrument was used for analysis of volatile organic compounds (VOCs) within exhaled breath samples. All study participants had undergone upper gastrointestinal endoscopy on the day of breath sampling. Receiver operating characteristic analysis and a diagnostic risk prediction model were used to assess the discriminatory accuracy of the identified VOCs.Results:Exhaled breath samples were analyzed from 81 patients with esophageal (N=48) or gastric adenocarcinoma (N=33) and 129 controls including Barrett's metaplasia (N=16), benign upper gastrointestinal diseases (N=62), or a normal upper gastrointestinal tract (N=51). Twelve VOCspentanoic acid, hexanoic acid, phenol, methyl phenol, ethyl phenol, butanal, pentanal, hexanal, heptanal, octanal, nonanal, and decanalwere present at significantly higher concentrations (P