Ascorbic Acid Integrates the Antagonistic Modulation of Ethylene and Abscisic Acid in the Accumulation of Reactive Oxygen Species
Ascorbic Acid Integrates the Antagonistic Modulation of Ethylene and Abscisic Acid in the Accumulation of Reactive Oxygen Species
复制标题
抗坏血酸将乙烯和脱落酸的拮抗调节作用整合到活性氧的积累中
DOI：
10.1104/pp.18.01250
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发表时间：
2019-04-01
期刊：
影响因子：
7.4
通讯作者：
Huang, Rongfeng
中科院分区：
文献类型：
--
作者：
Yu, Yanwen;Wang, Juan;Huang, Rongfeng
在植物生长发育过程中，乙烯和脱落酸（阿坝）发挥着重要作用，对多种生物学过程具有协同或拮抗作用，但这两种激素相互作用的具体机制，特别是对活性氧（ROS）积累的调控机制尚不清楚。在这里，我们报告，乙烯抑制，但阿坝促进积累的活性氧在拟南芥（拟南芥）幼苗。此外，抗坏血酸（阿萨）的生物合成的变化作为一个关键因素，整合乙烯和阿坝的相互作用，在ROS水平的调节。我们发现乙烯和阿坝分别通过乙烯不敏感因子3（EIN 3）和阿坝不敏感因子4（ABI 4）拮抗阿萨的生物合成，EIN 3和ABI 4分别是乙烯和阿坝信号途径的关键因子。此外，ABI 4在乙烯调节的阿萨生物合成中被EIN 3转录抑制。通过转录组分析和分子及遗传实验，我们确定维生素C缺陷2是ABI 4调节阿萨生物合成和ROS积累的直接靶点。因此，EIN 3-ABI 4-维生素C缺陷2转录级联反应涉及乙烯和阿坝拮抗调节阿萨生物合成和ROS积累以响应复杂环境刺激的机制。
During plant growth and development, ethylene and abscisic acid (ABA) play important roles and exert synergistic or antagonistic effects on various biological processes, but the detailed mechanism underlying the interaction of the two phytohormones, especially in the regulation of the accumulation of reactive oxygen species (ROS), is largely unclear. Here, we report that ethylene inhibits but ABA promotes the accumulation of ROS in Arabidopsis (Arabidopsis thaliana) seedlings. Furthermore, changes in the biosynthesis of ascorbic acid (AsA) act as a key factor in integrating the interaction of ethylene and ABA in the regulation of ROS levels. We found that ethylene and ABA antagonistically regulate AsA biosynthesis via ETHYLENE-INSENSITIVE3 (EIN3) and ABA INSENSITIVE4 (ABI4), which are key factors in the ethylene and ABA signaling pathways, respectively. In addition, ABI4 is transcriptionally repressed by EIN3 in ethylene-regulated AsA biosynthesis. Via transcriptome analysis and molecular and genetic experiments, we identified VITAMIN C DEFECTIVE2as the direct target of ABI4 in the regulation of AsA biosynthesis and ROS accumulation. Thus, the EIN3-ABI4- VITAMIN C DEFECTIVE2 transcriptional cascade involves a mechanism by which ethylene and ABA antagonistically regulate AsA biosynthesis and ROS accumulation in response to complex environmental stimuli.