Effects of chemical restraint on electroretinograms recorded sequentially in awake, sedated, and anesthetized dogs
Effects of chemical restraint on electroretinograms recorded sequentially in awake, sedated, and anesthetized dogs
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DOI：
10.2460/ajvr.74.7.1036
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发表时间：
2013-07-01
影响因子：
1
通讯作者：
Ofri, Ron
中科院分区：
文献类型：
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作者：
Freeman, Kate S.;Good, Kathryn L.;Ofri, Ron
定量和定性比较清醒，镇静和麻醉狗的视网膜电图（ERG）记录。动物-六只6个月大的比格犬。程序-使用狗的简要ERG协议。在1分钟和随后的5分钟暗适应后，使用手持式多物种ERG装置记录双侧混合视杆-视锥反应，犬处于3种意识状态：清醒、镇静（右美托咪定和布托啡诺）和麻醉（阿托品和氢吗啡酮，随后是丙泊酚和咪达唑仑以及异氟烷麻醉维持）。低频和高频噪声水平通过傅立叶分析进行量化，意识状态对信号幅度，内隐时间和噪声的影响通过重复测量方差分析进行分析。此外，13个兽医眼科医生谁不知道狗的意识状态主观分级的ERG记录质量，和分数为每个tracingcompared.Results-ERG振幅最高清醒的狗和最低的麻醉狗。内隐时间最短的清醒狗和最长的麻醉狗。b波振幅和a波内隐时间的差异有显著性。低频和高频噪声水平在意识状态之间没有显著差异。此外，没有显着差异，观察员的分数分配给ERG tracings.Conclusions和临床相关性麻醉和镇静导致狗的ERG反应显着衰减和延迟。狗的化学约束对低频或高频噪声水平或观察者对信号质量的感知没有持续的显著影响
Objective-To quantitatively and qualitatively compare electroretinography (ERG) recordings in awake, sedated, and anesthetized dogs.Animals-Six 6-month-old Beagles.Procedures-A brief ERG protocol for dogs was used. Following 1-minute and subsequent 5-minute dark adaptation, mixed rod-cone responses were recorded bilaterally with a handheld multispecies ERG device with dogs in each of 3 states of consciousness: awake, sedated (dexmedetomidine and butorphanol), and anesthetized (atropine and hydromorphone, followed by propofol and midazolam and anesthetic maintenance with isoflurane). Low- and high-frequency noise levels were quantified via Fourier analysis, and the effect of consciousness state on signal amplitude, implicit time, and noise was analyzed via repeated-measures ANOVA. In addition, 13 veterinary ophthalmologists who were unaware of the dogs' consciousness states subjectively graded the ERG recording quality, and scores for each tracing were compared.Results-ERG amplitudes were highest in awake dogs and lowest in anesthetized dogs. Implicit times were shortest in awake dogs and longest in anesthetized dogs. Differences in b-wave amplitudes and a-wave implicit times were significant. Neither low- nor high-frequency noise levels differed significantly among consciousness states. Furthermore, no significant differences were identified among observers' scores assigned to ERG tracings.Conclusions and Clinical Relevance-Anesthesia and sedation resulted in significant attenuation and delay of ERG responses in dogs. Chemical restraint of dogs had no consistently significant effect on low- or high-frequency noise levels or on observer perception of signal quality