Replication of varicella-zoster virus in human skin organ culture.
Replication of varicella-zoster virus in human skin organ culture.
复制标题
水痘带状疱疹病毒在人体皮肤器官培养物中的复制。
DOI：
10.1128/jvi.79.17.11501-11506.2005
复制
发表时间：
2005
影响因子：
5.4
通讯作者：
Moffat,JenniferF
中科院分区：
文献类型：
--
作者：
Taylor,ShannonL;Moffat,JenniferF
水痘-带状疱疹病毒(VZV)的感染仅限于人类，这阻碍了在啮齿动物疾病模型中对其发病机制的研究。为了便于研究VZV的皮肤嗜性，我们利用人胎儿皮肤器官培养(SOC)建立了一套体外系统。用空斑分析、透射电子显微镜和组织学方法分析VZV复制情况。感染水痘病毒的产量在6天内增加到∼的100倍，病毒粒子丰富，形成含有水痘和带状疱疹病毒抗原的皮损。VZV复制的SOC系统在测试病毒突变体和抗病毒药物方面有应用。
Varicella-zoster virus (VZV) infection is restricted to humans, which hinders studies of its pathogenesis in rodent models of disease. To facilitate the study of VZV skin tropism, we developed an ex vivo system using human fetal skin organ culture (SOC). VZV replication was analyzed by plaque assay, transmission electron microscopy, and histology. The yield of infectious VZV from SOC increased ∼100-fold over 6 days, virions were abundant, and lesions developed that contained VZV antigens and resembled varicella and zoster lesions. The SOC system for VZV replication has applications for testing virus mutants and antiviral drugs.