Low-frequency dynamics of biological molecules studied by terahertz time-domain spectroscopy
Low-frequency dynamics of biological molecules studied by terahertz time-domain spectroscopy
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DOI：
10.1155/2010/536453
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发表时间：
2010-01-01
影响因子：
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通讯作者：
Tominaga, Keisuke
中科院分区：
文献类型：
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作者：
Kawaguchi, Shintaro;Kambara, Ohki;Tominaga, Keisuke
用太赫兹(THz)时域谱测量了氨基酸(甘氨酸：Gly)、短肽((Gly)(3)和(Gly)(4))、六种球状蛋白和细菌视紫红质(BR)的低频光谱。通过对THz谱的分析，我们定义并得到了这些情况下的约化吸收截面，它们与态的振动密度成正比。我们观察到了短肽在低频模式下的非谐行为。我们研究的球状蛋白在低频光谱中显示出一个普遍的特征。当样品水化时，BR显示了THz谱随温度变化的动态转变。
By terahertz (THz) time-domain spectroscopy we have measured low-frequency spectra of amino acid (glycine: Gly), short peptides ((Gly)(3) and (Gly)(4)), six globular proteins and bacteriorhodopsin (BR). From the analysis of the THz spectra we defined and obtained the reduced absorption cross sections for these cases, which are proportional to the vibrational density of states. We observed anharmonic behaviors in the low-frequency modes of the short peptides. The globular proteins we investigated show a universal feature in the low-frequency spectra. BR shows the dynamical transition in the temperature dependence of the THz spectrum when the sample is hydrated.