Two-photon AgNP/DNA-TP dye nanosensing conjugate for biothiol probing in live cells.
Two-photon AgNP/DNA-TP dye nanosensing conjugate for biothiol probing in live cells.
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DOI：
10.1039/c4an01381f
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发表时间：
2014-10
期刊：
影响因子：
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通讯作者：
Mingli Liu;Qiao Tang;Ting Deng;Huijuan Yan;Jishan Li;Yinhui Li;Ronghua Yang
中科院分区：
文献类型：
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作者：
Mingli Liu;Qiao Tang;Ting Deng;Huijuan Yan;Jishan Li;Yinhui Li;Ronghua Yang
制备了一种新型的银纳米粒子（AgNP）/DNA-双光子染料（TP dye）偶联物作为双光子纳米探针用于活细胞中的生物硫醇成像。以DNA为模板的银纳米颗粒是有效的猝灭剂，并且还提供了用于将DNA容易地递送到活细胞中的生物相容性纳米平台。在存在生物硫醇（Cys、Hcy或GSH）的情况下，硫醇基团与银之间的强相互作用导致TP染料标记的单链DNA（ssDNA）从AgNP表面释放，随后TP染料发射荧光，从而能够测定生物硫醇。我们的研究结果表明，AgNP/DNA-TP染料nanosensing共轭物不仅是一个强大的，灵敏的，和选择性的传感器，用于定量检测生物硫醇在复杂的生物环境中，但也可以有效地传递到活细胞，并作为一个“信号”传感器的目标生物分子的特异性，高对比度成像。我们的设计提供了一种方法，为未来的DNA模板的银纳米粒子为基础的双光子荧光探针，用于在体外或体内作为生物分子传感器活细胞成像的发展。
A novel silver nanoparticle (AgNP)/DNA-two-photon dye (TP dye) conjugate was fabricated as a two-photon nanoprobe for biothiol imaging in live cells. DNA-templated silver nanoparticles are efficient quenchers and also provide a biocompatible nanoplatform for facile delivery of DNA into living cells. In the presence of biothiols (Cys, Hcy, or GSH), the strong interaction between the thiol group and silver results in the release of TP dye-labeled single-stranded DNA (ssDNA) from the AgNP surface and the subsequent fluorescence emission of the TP dye, thus enabling biothiols to be assayed. Our results reveal that the AgNP/DNA-TP dye nanosensing conjugate not only is a robust, sensitive, and selective sensor for quantitative detection of biothiols in the complex biological environment but also can be efficiently delivered into live cells and act as a "signal-on" sensor for specific, high-contrast imaging of target biomolecules. Our design provides a methodology for the development of future DNA-templated silver nanoparticle-based two-photon fluorescent probes for use in vitro or in vivo as biomolecular sensors for live-cell imaging.