PREFERENTIAL DIGESTION OF BASEMENT-MEMBRANE COLLAGEN BY AN ENZYME DERIVED FROM A METASTATIC MURINE TUMOR
PREFERENTIAL DIGESTION OF BASEMENT-MEMBRANE COLLAGEN BY AN ENZYME DERIVED FROM A METASTATIC MURINE TUMOR
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DOI：
10.1073/pnas.76.5.2268
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发表时间：
1979-01-01
影响因子：
11.1
通讯作者：
MARTIN, GR
中科院分区：
文献类型：
--
作者：
LIOTTA, LA;ABE, S;MARTIN, GR
用Ⅰ、Ⅱ、Ⅲ、Ⅳ和V（AB）型胶原作为底物，研究了人皮肤胶原酶和一种侵袭性肿瘤酶的特异性。人皮肤胶原酶降解I、II和III型胶原，产生特征性的3/4和1/4裂解产物，但不能降解IV或V型胶原。从侵袭性肿瘤中制备的胶原酶在胰蛋白酶处理后表现出最大活性。肿瘤酶降解IV型（基底膜）胶原，产生与单个切割位点一致的片段，但不攻击I、II、III和V型胶原。因为通过胎盘的胃蛋白酶化制备的IV型胶原也被消化，所以肿瘤胶原酶对IV型胶原的切割可能发生在大部分螺旋结构域内。IV型胶原酶可在肿瘤转移和基底膜更新发生的正常组织中发挥重要作用。
The specificity of human skin collagenase and of an enzyme from an invasive tumor were studied by using types I, II, III, IV and V (AB) collagen as substrates. Human skin collagenase degraded types I, II and III collagen, producing the characteristic 3/4 and 1/4 cleavage products, but failed to degrade type IV or V collagen. Collagenase prepared from the invasive tumor showed maximal activity after trypsin treatment. The tumor enzyme degraded type IV (basement membrane) collagen, producing fragments consistent with a single cleavage site but did not attack types I, II, III and V collagen. Because type IV collagen prepared by pepsinization of placenta was also digested, cleavage of type IV collagen by the tumor collagenase may occur within a largely helical domain. A type IV collagenase could play a significant role in tumor metastases and in normal tissues where basement membrane turnover takes place.