Serological and Molecular Detection of Senecavirus A Associated with an Outbreak of Swine Idiopathic Vesicular Disease and Neonatal Mortality
Serological and Molecular Detection of Senecavirus A Associated with an Outbreak of Swine Idiopathic Vesicular Disease and Neonatal Mortality
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DOI：
10.1128/jcm.00710-16
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发表时间：
2016-08-01
影响因子：
9.4
通讯作者：
Pineyro, Pablo
中科院分区：
文献类型：
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作者：
Gimenez-Lirola, Luis Gabriel;Rademacher, Chris;Pineyro, Pablo
我们在一个表现为水泡病(VD)暴发的种猪群中进行了一项纵向实地研究，这与新生儿死亡率的增加有关。最初，美国农业部的外来动物疾病(FAD)调查证实存在SenecaVirus A(SVA)，并排除了产生水泡病变的外来病原体的存在，例如口蹄疫病毒和其他病毒。随后，从临床暴发后1周开始收集母猪(n=22)及其仔猪(n=33)的血清样本、扁桃体拭子和粪便，每周收集一次，为期6周。用逆转录定量聚合酶链式反应(RT-qPCR)检测SVA RNA在5‘非翻译区的保守区的存在。用SVA VP1重组蛋白(RVP1)间接酶联免疫吸附试验(ELISA法)每周检测母猪和仔猪血清样品对SVA的血清学应答(Ig G)。RVP1-EL ISA检测了暴发早期临床感染和非临床感染母猪的SVA血清转换，以及后代中的母体SVA抗体。总体而言，在SVA感染的动物中没有水泡(大体病变)，而且RT-qPCR结果在标本类型之间存在差异，这表明基于临床观察、多个诊断标本的RT-qPCR和血清学相结合的诊断算法对于确保SVA的准确诊断至关重要。
We performed a longitudinal field study in a swine breeding herd that presented with an outbreak of vesicular disease (VD) that was associated with an increase in neonatal mortality. Initially, a USDA Foreign Animal Disease (FAD) investigation confirmed the presence of Senecavirus A (SVA) and ruled out the presence of exotic agents that produce vesicular lesions, e.g., foot-and-mouth disease virus and others. Subsequently, serum samples, tonsil swabs, and feces were collected from sows (n = 22) and their piglets (n = 33) beginning 1 week after the onset of the clinical outbreak and weekly for 6 weeks. The presence of SVA RNA was evaluated in all specimens collected by reverse transcriptase quantitative PCR (RT-qPCR) targeting a conserved region of the 5' untranslated region (5'-UTR). The serological response (IgG) to SVA was evaluated by the weekly testing of sow and piglet serum samples on a SVA VP1 recombinant protein (rVP1) indirect enzyme-linked immunosorbent assay (ELISA). The rVP1 ELISA detected seroconversion against SVA in clinically affected and non-clinically affected sows at early stages of the outbreak as well as maternal SVA antibodies in offspring. Overall, the absence of vesicles (gross lesions) in SVA-infected animals and the variability of RT-qPCR results among specimen type demonstrated that a diagnostic algorithm based on the combination of clinical observations, RT-qPCR in multiple diagnostic specimens, and serology are essential to ensure an accurate diagnosis of SVA.