Changes in muscle fiber type and expression of mRNA of myosin heavy chain isoforms in porcine muscle during pre- and postnatal development
Changes in muscle fiber type and expression of mRNA of myosin heavy chain isoforms in porcine muscle during pre- and postnatal development
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DOI：
10.1111/asj.12641
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发表时间：
2017-02-01
影响因子：
2
通讯作者：
Ashihara, Akane
中科院分区：
文献类型：
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作者：
Katsumata, Masaya;Yamaguchi, Tomomi;Ashihara, Akane
本研究旨在阐明猪在出生前后肌纤维类型的发育变化。为此，我们进行了肌球蛋白腺苷三磷酸酶活性的组织化学分析，以评估肌纤维的类型和确定的信使RNA（mRNA）的肌球蛋白重链（MHC）亚型的丰度。在胎儿期第90天从胎儿中采集背最长肌（LD）样本。此外，LD、菱形肌和股二头肌（B.分别于1、12、26、45和75日龄取猪股肌。LD和B区MHC 2 B mRNA的表达。从胎儿晚期到出生后早期，股肌的数量迅速而显著地增加，这种增加至少在LD肌中与2b型纤维的发育有关。MHC2b mRNA表达的快速而显著的变化表明，在此发育阶段，肌纤维类型仍具有一定的可塑性。
The purpose of this study is to elucidate developmental changes in muscle fiber type in the pig during pre- and postnatal development. For this purpose, we performed a histochemical analysis for myosin adenosine triphosphatase activity to assess muscle fiber type and determined abundances of messenger RNA (mRNA) of myosin heavy chain (MHC) isoforms. Samples of Longissimus dorsi (LD) muscle were taken from fetuses on day 90 of the fetal stage. Further, samples of LD, Rhomboideus and Biceps femoris (B. femoris) muscles were taken from pigs when they were 1, 12, 26, 45 or 75days old. Expression of MHC 2b mRNA in the LD and the B. femoris muscles rapidly and considerably increased from the late fetal stage to the early postnatal stage and this increase was associated with the development of type 2b fibers at least in the LD muscle. As shown by the rapid and considerable changes in expression of MHC 2b mRNA, it seems that a certain plasticity of muscle fiber type still remains in this developmental stage.