Covalently incorporated protein-nanogels using AGET ATRP in an inverse miniemulsion
Covalently incorporated protein-nanogels using AGET ATRP in an inverse miniemulsion
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DOI：
10.1039/c1py00050k
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发表时间：
2011-01-01
影响因子：
4.6
通讯作者：
Matyjaszewski, Krzysztof
中科院分区：
文献类型：
--
作者：
Averick, Saadyah E.;Magenau, Andrew J. D.;Matyjaszewski, Krzysztof
以含有非天然氨基酸的基因工程蛋白为原料，采用原子转移自由基聚合（ATRP）引发剂，在反相微乳液中通过电子转移自由基聚合（AGET）激活剂合成了蛋白质-纳米凝胶杂化体（PNHs）。所提出的路线是一种适当的合成策略，共价和位点特异性地将绿色荧光蛋白（GFP）结合到定义良好的纳米凝胶中。利用动态光散射（DLS）、紫外-可见荧光光谱和共聚焦显微镜对这些PNHs进行分析，以确认GFP蛋白成功整合到每个纳米凝胶（NG）中，同时保留其原生三级结构。
Using a genetically engineered protein, containing a non-natural amino acid bearing an atom transfer radical polymerization (ATRP) initiator, protein-nanogel hybrids (PNHs) were synthesized by activator generated by electron transfer (AGET) ATRP in an inverse miniemulsion. The route presented is an appropriate synthetic strategy to covalently and site specifically incorporate green fluorescent protein (GFP) into well-defined nanogels. These PNHs were analyzed using dynamic light scattering (DLS), UV-visible fluorescence spectroscopy and confocal microscopy to confirm the successful integration of GFP proteins into each nanogel (NG), while preserving its native tertiary structure.