DEVELOPMENTAL REGULATION OF CONNEXIN40 GENE-EXPRESSION IN MOUSE HEART CORRELATES WITH THE DIFFERENTIATION OF THE CONDUCTION SYSTEM
DEVELOPMENTAL REGULATION OF CONNEXIN40 GENE-EXPRESSION IN MOUSE HEART CORRELATES WITH THE DIFFERENTIATION OF THE CONDUCTION SYSTEM
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DOI：
10.1002/aja.1002040403
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发表时间：
1995-12-01
影响因子：
2.5
通讯作者：
THEVENIAU-RUISSY, M
中科院分区：
文献类型：
--
作者：
DELORME, B;DAHL, E;THEVENIAU-RUISSY, M
在成年小鼠心脏中，CX 40在心房和心室传导系统的近端部分（希氏束和束支的上部）表达。这种心脏组织专门负责电脉冲的传导。CX40是已知在成人传导组织的这些部分中表达的唯一小鼠连接蛋白，因此被认为是传导系统的标志物。在本报告中，我们研究了CX40在小鼠心脏发育过程中的表达和分布。我们首先证明，CX 40 mRNA在整个发育过程中受到调控，其他心脏连接蛋白转录本也是如此，即，CX 37、CX 43和CX 45，随着开发的进行，丰度逐渐减少。我们还表明，CX40的转录和蛋白质类似地调节，CX40被表达为两种不同的磷酸化和非磷酸化形式的41和40 kDa，分别。令人惊讶的是，分布研究表明，CX40广泛表达在11天后coclonal（dpc）的胚胎心脏，它是在心房和心室原基检测。随着发育的进行，CX40在心房中的分布模式得以维持，而在心室中观察到更动态的模式。从14 dpc开始，随着成人心室传导系统的分化，CX40在小梁网络中减少，并优先分布在心室传导系统中。因此，CX40是早期分化传导系统的标志物。假设传导系统在11 dpc时以无组织的“胚胎”形式存在，并在14 dpc时转分化为成人传导系统。(C)1995 Wiley-Liss，Inc.
In adult mouse heart, CX40 is expressed in the atria and the proximal part of the ventricular conduction system (the His bundle and the upper parts of the bundle branches). This cardiac tissue is specialized in the conduction of the electrical impulse. CX40 is the only mouse connexin known to be expressed in these parts of the adult conductive tissue and is thus considered as a marker of the conduction system. In the present report, we investigated CX40 expression and distribution during mouse heart development. We first demonstrate that CX40 mRNA is regulated throughout development, as are other heart connexin transcripts, i.e., CX37, CX43, and CX45, with a decreasing abundance as development proceeds. We also show that the CX40 transcript and protein are similarly regulated, CX40 being expressed as two different phosphorylated and un-phosphorylated forms of 41 and 40 kDa, respectively. Surprisingly, distribution studies demonstrated that CX40 is widely expressed in 11 days post-coitum (dpc) embryonic heart, where it is detected in both the atria and ventricle primordia. As development proceeds, the CX40 distribution pattern in the atria is maintained, whereas a more dynamic pattern is observed in the ventricles. From 14 dpc onwards, as the adult ventricular conduction system differentiates, CX40 decreases in the trabecular network and it is preferentially distributed in the ventricular conduction system. CX40 is thus the marker of the early differentiating conduction system. It is hypothesized that the conduction system is present in unorganized ''embryonic'' form at 11 dpc and trans-differentiates by 14 dpc into the adult conduction system. (C) 1995 Wiley-Liss, Inc.