PpVIN2, an acid invertase gene family member, is sensitive to chilling temperature and affects sucrose metabolism in postharvest peach fruit
PpVIN2, an acid invertase gene family member, is sensitive to chilling temperature and affects sucrose metabolism in postharvest peach fruit
复制标题
PpVIN2是酸性转化酶基因家族成员，对冷藏温度敏感并影响采后桃果实的蔗糖代谢
DOI：
10.1007/s10725-018-0419-z
复制
发表时间：
2018-11-01
影响因子：
4.2
通讯作者：
Shao, Xingfeng
中科院分区：
文献类型：
--
作者：
He, Xingxing;Wei, Yingying;Shao, Xingfeng
我们之前报道过，在低温胁迫下，桃果实中酸性转化酶（AI）的表达和活性增加。为了确定哪些 AI 基因对冷胁迫有反应，鉴定并克隆了 7 个 AI 基因，即 2 个液泡转化酶 (VIN) 和 5 个细胞壁结合转化酶 (CWIN)。预测的基因氨基酸序列包含植物 AI 特有的保守位点，例如 NDPNG/A（蔗糖结合位点）和 MWECV/P（半胱氨酸催化基序）。使用基因特异性引物，测量了储存在 0、5、10 和 20°C 的‘白凤’和‘玉露’桃果实中每个基因的表达。 7个基因中，PpVIN2的表达受冷胁迫影响最大；增幅最大的是在 5°C 下储存的水果，“白丰”水果增加了 17 倍，“玉露”水果增加了 280 倍。总体而言，贮藏桃果中的 VIN 活性远高于 CWIN 活性。 5℃贮藏期间，与其他温度相比，两个品种的还原糖含量均显着增加，蔗糖含量逐渐下降，并伴有严重的冷害症状。因此，PpVIN2似乎是由低温诱导的，并且可能在冷藏桃果实的蔗糖代谢中发挥重要作用。
We previously reported that expression and activity of acid invertases (AI) are increased in peach fruit under chilling stress. In order to determine which AI genes respond to chilling stress, seven AI genes, two vacuolar invertases (VINs) and five cell wall-bound invertases (CWINs), were identified and cloned. The predicted amino acid sequences of the genes contain conserved sites characteristic of plant AIs such as NDPNG/A, the sucrose-binding site, and MWECV/P, a cysteine catalytic motif. Using gene-specific primers, the expression of each gene was measured in ‘Baifeng’ and ‘Yulu’ peach fruits stored at 0, 5, 10 and 20 °C. Of the seven genes, expression ofPpVIN2was the most affected by chilling stress; the largest increases were in fruit stored at 5 °C, up to 17-fold in ‘Baifeng’ fruit, and up to 280-fold in ‘Yulu’ fruit. Overall, VIN activity was much higher than CWIN activity in stored peach fruit. In both cultivars reducing sugar content increased significantly and sucrose content decreased gradually during storage at 5 °C relative to other temperatures, and was accompanied by severe chilling injury symptoms. Thus,PpVIN2appears to be induced by chilling and may play an important role in sucrose metabolism in peach fruit subjected to cold storage.