Correlation of alternative splicing of the D2 dopamine receptor mRNA and estrogen receptor mRNA in the prolactinomas and gonadotrope tumors
Correlation of alternative splicing of the D2 dopamine receptor mRNA and estrogen receptor mRNA in the prolactinomas and gonadotrope tumors
复制标题
DOI：
10.1007/s11060-009-9816-5
复制
发表时间：
2009-02
影响因子：
3.9
通讯作者：
Zhe-bao Wu;Chu Zhong Li;Xu-yi Zong;Z. Su;Yan Zeng;Ya Zhuo Zhang
中科院分区：
文献类型：
--
作者：
Zhe-bao Wu;Chu Zhong Li;Xu-yi Zong;Z. Su;Yan Zeng;Ya Zhuo Zhang
雌二醇（E2）通过核雌激素受体（ER）调节两种多巴胺D2受体亚型（D2 L/D2 S）的比例，并降低多巴胺对PRL分泌的抑制作用。本研究旨在探讨ER mRNA和D2 R mRNA在垂体腺瘤细胞中表达的相关性。采用半定量RT-PCR方法检测14例泌乳素腺瘤和10例促性腺激素肿瘤中ERβ mRNA和D2 R mRNA的表达ERα mRNA表达与正常对照组比较差异有统计学意义（P= 0.003）。D2 S和D2 LmRNA在两种类型垂体腺瘤中的表达差异有统计学意义（P值分别为0.036和0.007）。此外，泌乳素瘤的表达水平均显著高于促性腺细胞肿瘤。D2 S与ERα mRNA表达呈负相关，D2 L与ERα mRNA表达呈正相关。结论本研究首次发现泌乳素腺瘤和促性腺激素肿瘤中ER和D2 R亚型表达之间存在良好的相关性。降低垂体腺瘤细胞中ERα的含量可改变D2 L/D2 S的比值，从而增加垂体腺瘤的药物敏感性。
ObjectiveEstradiol (E2) acts to modulate the ratio of two dopamine D2 receptor isoforms (D2L/D2S) by the nuclear estrogen receptor (ER) and to reduce dopamine’s inhibitory action on PRL secretion. Here we demonstrate the correlation between the expression of ER mRNA and D2R mRNA isoforms in pituitary neoplasms cells.MethodsTwenty-four human pituitary adenomas (14 prolactinomas and 10 gonadotrope tumors) were examined for the expression of both ER mRNA and D2R mRNA by means of semi-quantitative RT-PCR analysis.ResultsNo significant difference was found in ERβ mRNA expression levels between prolactinomas and gonadotrope tumors (P= 0.871), but there was a significant difference in the expression of ERα mRNA (P= 0.003). The significant difference was found between the two pituitary adenomas types in both levels of D2S and D2L mRNA expression (P= 0.036 and 0.007 respectively). Furthermore, both levels of expression in prolactinomas were significantly higher than that in gonadotrope tumors. Additionally, a negative correlation between D2S and ERα mRNA expression and a positive correlation between D2L and ERα mRNA expression were found in these tumors.ConclusionThis study for the first time shows a good correlation between expression of ER and D2R isoforms in prolactinomas and gonadotrope tumors. Reducing the amount of the ERα in neoplasm cells can alter the ratio of D2L/D2S, which may increase the drug sensitivity of pituitary adenomas.