Electrochemical immunoanalysis for carcinoembryonic antigen based on multilayer architectures of gold nanoparticles and polycation biomimetic interface on glassy carbon electrode
Electrochemical immunoanalysis for carcinoembryonic antigen based on multilayer architectures of gold nanoparticles and polycation biomimetic interface on glassy carbon electrode
复制标题
基于金纳米粒子多层结构和玻碳电极聚阳离子仿生界面的癌胚抗原电化学免疫分析
DOI：
10.1002/elan.200603700
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发表时间：
2006-12-01
期刊：
影响因子：
3
通讯作者：
Peng, Yungui
中科院分区：
文献类型：
--
作者：
Fu, Yingzi;Yaan, Ruo;Peng, Yungui
本文介绍了壳聚糖(CTS)和金纳米颗粒(Au)在经过处理的负电荷玻碳(GC)电极上的逐层自组装过程，制备了具有无毒仿生界面的电化学免疫传感器，为固定化抗癌胚抗体(anti-CEA)监测癌胚抗原(CEA)提供了类似天然体系的环境和更大的定位自由度。用UV-Vis光谱、原子力显微镜(AFM)和循环伏安(CV)测量来跟踪多层膜的形成。详细研究了Biominetic界面的性能和影响检测系统的因素。CEA浓度测定采用微分脉冲伏安法(DPV)电流响应法。该方法的动态范围为0.5 0~80.0 0 ng m L−1，检测下限为0.2 7 ng m L−1，检测时间为3σ。实验结果表明，该固定化方法具有良好的灵敏度、选择性和稳定性。
This paper describes a layer-by-layer (LBL) self-assembly process of chitosan (CTS) and gold nanoparticles (Au) on the pretreated negatively charged glassy carbon (GC) electrode to fabricate electrochemistry immunosensor with a nontoxic biomimetic interface, which provided an environment similar to a native system and allowed more freedom in orientation for immobilization of carcinoembryonic antibody (anti-CEA) to monitor carcinoembryonic antigen (CEA). UV-vis spectroscope, atomic force microscopy (AFM), and cyclic voltammetric (CV) measurements were used to follow the multilayer film formation. The performance of the biominetic interface and factors influencing the assay system were investigated in detail. The differential pulse voltammetry (DPV) current response is used for the CEA concentration assay. The dynamic range was from 0.50 to 80.00 ng mL−1 with a detection limit of 0.27 ng mL−1 at 3σ. In addition, the experiment results indicate that immobilization described in this proposed method exhibits a good sensitivity, selectivity, and stability.