ABA inhibits entry into stomatal-lineage development in Arabidopsis leaves
ABA inhibits entry into stomatal-lineage development in Arabidopsis leaves
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DOI：
10.1111/tpj.12136
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发表时间：
2013-05-01
期刊：
影响因子：
7.2
通讯作者：
Kamiya, Yuji
中科院分区：
文献类型：
--
作者：
Tanaka, Yoko;Nose, Tomoe;Kamiya, Yuji
气孔的数量和密度受内源和环境因素的控制。尽管最近的进展，我们的理解气孔发育，防止气孔谱系进入的机制仍然不清楚。在这里，我们建议，脱落酸（ABA），已知诱导气孔关闭的植物激素，限制气孔发育的启动，并诱导扩大路面细胞在拟南芥子叶。ABA缺陷型aba2 - 2突变体在较小的子叶内具有增加的气孔数量/比例，以及减少的铺面细胞的扩张。这种趋势被逆转后ABA的应用程序或在ABA过度积累cyp707a1 cyp707a3 doublemutant。我们的时间进程分析表明，aba2 - 2显示延长分生组织和保卫母细胞，气孔的前体形成。这一发现是根据延长的SPCH和MUTE，气孔形成的主调节基因的表达，表明ABA的行为这些基因的上游。只有aba2 - 2静音，但没有aba2 - 2 spch双突变体显示添加剂的表型和显示抑制路面细胞扩大与增加分生组织数量，表明ABA对路面细胞扩张的作用需要存在的气孔谱系细胞。
The number and density of stomata are controlled by endogenous and environmental factors. Despite recent advances in our understanding of stomatal development, mechanisms which prevent stomatal-lineage entry remain unclear. Here, we propose that abscisic acid (ABA), a phytohormone known to induce stomatal closure, limits initiation of stomatal development and induces enlargement of pavement cells in Arabidopsis cotyledons. An ABA-deficient aba2-2 mutant had an increased number/proportion of stomata within a smaller cotyledon, as well as reduced expansion of pavement cells. This tendency was reversed after ABA application or in an ABA over-accumulating cyp707a1cyp707a3 doublemutant. Our time course analysis revealed that aba2-2 shows prolonged formation of meristemoids and guard mother cells, both precursors of stoma. This finding is in accordance with prolonged gene expression of SPCH and MUTE, master regulators for stomatal formation, indicating that ABA acts upstream of these genes. Only aba2-2 mute, but not aba2-2 spch double mutant showed additive phenotypes and displayed inhibition of pavement cell enlargement with increased meristemoid number, indicating that ABA action on pavement cell expansion requires the presence of stomatal-lineage cells.