Diagnostic importance of CD179a/b as markers of precursor B-cell lymphoblastic lymphoma
Diagnostic importance of CD179a/b as markers of precursor B-cell lymphoblastic lymphoma
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DOI：
10.1038/modpathol.3800079
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发表时间：
2004-04-01
期刊：
影响因子：
7.5
通讯作者：
Fujimoto, J
中科院分区：
文献类型：
--
作者：
Kiyokawa, N;Sekino, T;Fujimoto, J
由VpreB(CD179a)和lambda5(CD179b)组成的替代轻链在缺乏完整免疫球蛋白的前体B细胞中表达，被认为是传统轻链的替代品。在分化为未成熟和成熟的B细胞后，CD179a/b消失，取而代之的是传统的轻链。因此，这些分子可能作为前体B细胞的基本标志物。为了检测代用品轻链成分CD179a和CD179b在前体B细胞淋巴母细胞淋巴瘤中的表达，我们用免疫组织化学技术分析了组织切片。在大量替代轻链的单抗中，VpreB8和SL11在丙酮固定的新鲜冰冻切片中分别检测到CD179a和CD179b。此外，我们还在福尔马林固定的石蜡包埋切片中观察到VpreB8染色。利用这些抗体，我们发现CD179a/b在前体B细胞淋巴母细胞淋巴瘤中特异表达，但在儿童成熟B细胞淋巴瘤中不表达。此外，其他必须包括在前体B细胞淋巴母细胞性淋巴瘤鉴别诊断中的儿科肿瘤，包括前体T细胞淋巴母细胞性淋巴瘤、髓外髓系肿瘤和尤文肉瘤，CD179a和CD179b也均为阴性。提示CD179a和CD179b可能是前体B细胞淋巴母细胞淋巴瘤免疫表型诊断的重要指标。
Surrogate light chains consisting of VpreB (CD179a) and lambda5 (CD179b) are expressed in precursor B cells lacking a complete form of immunoglobulin and are thought to act as substitutes for conventional light chains. Upon differentiation to immature and mature B cells, CD179a/b disappear and are replaced with conventional light chains. Thus, these molecules may be useful as essential markers of precursor B cells. To examine the expression of the surrogate light-chain components CD179a and CD179b in precursor B-cell lymphoblastic lymphoma, we analyzed tissue sections using immunohistochemistry techniques. Among a number of monoclonal antibodies for the surrogate light chains, VpreB8 and SL11 were found to detect CD179a and CD179b, respectively, in acetone-fixed fresh frozen sections. Moreover, we also observed VpreB8 staining in formalin-fixed, paraffin-embedded sections. Using these antibodies, we found that CD179a/b were specifically expressed in precursor B-cell lymphoblastic lymphomas, but not in mature B-cell lymphomas in childhood. Furthermore, other pediatric tumors that must be included in a differential diagnosis of precursor B-cell lymphoblastic lymphoma, including precursor T-cell lymphoblastic lymphoma, extramedullary myeloid tumors, and Ewing sarcoma, were also negative for both CD179a and CD179b. Our data indicate that CD179a and CD179b may be important markers for the immunophenotypic diagnosis of precursor B-cell lymphoblastic lymphomas.