Comprehensive interaction of dicalcin with annexins in frog olfactory and respiratory cilia
Comprehensive interaction of dicalcin with annexins in frog olfactory and respiratory cilia
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DOI：
10.1111/j.1742-4658.2007.06007.x
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发表时间：
2007-09
期刊：
影响因子：
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通讯作者：
T. Uebi;N. Miwa;S. Kawamura
中科院分区：
文献类型：
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作者：
T. Uebi;N. Miwa;S. Kawamura
Dicalcin（从p26 olf重新命名）是在青蛙嗅上皮中发现的S100蛋白的二聚体形式。S100蛋白是一组EF-手型钙离子结合蛋白，已知可与多种靶蛋白相互作用以改变其活性。为了确定二钙素在嗅上皮中的作用，我们鉴定了其结合蛋白。蛙嗅上皮中的几种蛋白质被发现以Ca 2+依赖的方式与二钙蛋白结合。其中38 kDa和35 kDa蛋白质含量最高。我们的分析表明，这些是膜联蛋白A1，膜联蛋白A2和膜联蛋白A5的混合物。免疫组织化学分析表明，二钙蛋白和所有这三种亚型的膜联蛋白共定位于嗅纤毛。发现二钙蛋白以几乎足以结合所有这些膜联蛋白的量存在。在蛙呼吸纤毛中也观察到双钙蛋白和膜联蛋白的三种亚型的共定位。Dicalcin促进由膜联蛋白A1或膜联蛋白A2引起的Ca 2+依赖性脂质体聚集，当膜联蛋白A1和膜联蛋白A2都存在时，这种促进作用是累加的。在这种易化作用中，annexin A1和annexin A2的有效Ca ~（2+）浓度不同，因此蛙嗅觉和呼吸纤毛中的双钙蛋白-annexin系统可以覆盖很宽的Ca ~（2+）浓度范围。这些结果表明，该系统与异常增加的Ca 2+浓度在嗅觉和其他运动纤毛。
Dicalcin (renamed from p26olf) is a dimer form of S100 proteins found in frog olfactory epithelium. S100 proteins form a group of EF‐hand Ca2+‐binding proteins, and are known to interact with many kinds of target protein to modify their activities. To determine the role of dicalcin in the olfactory epithelium, we identified its binding proteins. Several proteins in frog olfactory epithelium were found to bind to dicalcin in a Ca2+‐dependent manner. Among them, 38 kDa and 35 kDa proteins were most abundant. Our analysis showed that these were a mixture of annexin A1, annexin A2 and annexin A5. Immunohistochemical analysis showed that dicalcin and all of these three subtypes of annexin colocalize in the olfactory cilia. Dicalcin was found to be present in a quantity almost sufficient to bind all of these annexins. Colocalization of dicalcin and the three subtypes of annexin was also observed in the frog respiratory cilia. Dicalcin facilitated Ca2+‐dependent liposome aggregation caused by annexin A1 or annexin A2, and this facilitation was additive when both annexin A1 and annexin A2 were present. In this facilitation effect, the effective Ca2+ concentrations were different between annexin A1 and annexin A2, and therefore the dicalcin–annexin system in frog olfactory and respiratory cilia can cover a wide range of Ca2+ concentrations. These results suggested that this system is associated with abnormal increases in the Ca2+ concentration in the olfactory and other motile cilia.