Electrophoretic and serological analyses of cytoplasmic antigens from Aspergillus fumigatus during growth of conidia to mature mycelia.
Electrophoretic and serological analyses of cytoplasmic antigens from Aspergillus fumigatus during growth of conidia to mature mycelia.
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DOI：
10.1080/02681218780000581
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发表时间：
1987-07
期刊：
影响因子：
--
通讯作者：
J. Piechura;R. S. Riefel;L. Daft
中科院分区：
文献类型：
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作者：
J. Piechura;R. S. Riefel;L. Daft
利用一、二维聚丙烯酰胺凝胶电泳法分析了烟曲霉Ag507菌株成熟菌丝生长过程中伴随分生孢子的细胞质成分的变化。SDS-PAGE检测分生孢子和菌丝生长96h得到的胞浆各组分的相对分子质量差异。2-DE分析表明，成熟胞浆的某些特征成分在7h后开始出现。将兔免疫球蛋白提纯到成熟胞浆中，对吸收的胞浆样品进行2-DE分析。与菌丝生长后期不同的是，分生孢子胞质组分没有被很大程度地吸收，这表明在真菌生长过程中胞质组分可能会发生变化和/或从头合成。融合火箭免疫电泳法分析表明，在生长过程中的不同时间，某些成分的合成量不同：生长3、4、7、8和18h，开始出现可能从头合成的成分。用兔抗成熟胞浆抗血清和从分生孢子经96h生长获得的胞浆样品进行酶联免疫吸附试验，表明胞浆样品的分子结构存在差异。变态反应性支气管肺曲霉菌病患者血清中抗细胞溶血素Ig G和Ig E效价均升高，并随制剂的不同而波动。从生长4、5、7、9h获得的胞浆制剂，其特异性Ig G和Ig E效价均呈升高趋势，以96h最高。
The changes of cytoplasmic components concomitant with conidium to mature mycelium growth of Aspergillus fumigatus strain Ag 507 were analysed by one- and two-dimensional polyacrylamide gel electrophoresis (SDS-PAGE; 2-DE). SDS-PAGE monitored molecular weight differences between components of cytosol preparations obtained from conidia and those through 96 h of mycelial growth. 2-DE analyses indicated that some components characteristic of mature cytosol begin to appear by 7 h. Cytoplasmic preparations absorbed with rabbit immunoglobulins raised to mature cytosol were analysed by 2-DE. Conidia cytosol components were not absorbed to a great degree, unlike those from later stages of mycelial growth, which indicates that cytosol components may be changed and/or synthesized de novo during growth of the fungus. Analysis of the cytosol preparations by fused rocket immunoelectrophoresis showed that some components are synthesized in different amounts at various times during growth: 3, 4, 7, 8, and 18 h of growth, components begin to appear that may be synthesized de novo. Enzyme-linked immunosorbent assay with rabbit antiserum to mature cytosol and cytosol preparations obtained from conidia through 96 h of growth, indicated differences of molecular structures between the cytosol preparations. The anticytosol IgG and IgE titers of sera from patients with allergic bronchopulmonary aspergillosis were both elevated and fluctuated with each preparation. The specific IgG and IgE titers both appeared to be elevated with cytosol preparations obtained from 4, 5, 7, and 9 h of growth and highest against the 96 h preparation.