Nanotool exchanger system based on E-SEM nanorobotic manipulation system
Nanotool exchanger system based on E-SEM nanorobotic manipulation system
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DOI：
10.1109/icra.2012.6225333
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发表时间：
2012-05
期刊：
影响因子：
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通讯作者：
M. Nakajima;T. Kawamoto;T. Hirano;M. Kojima;T. Fukuda
中科院分区：
文献类型：
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作者：
M. Nakajima;T. Kawamoto;T. Hirano;M. Kojima;T. Fukuda
基于环境扫描电子显微镜（E-SEM）纳米机器人操作系统，提出了一种新型的纳米工具交换器系统。我们提出了使用E-SEM纳米操纵系统的生物样品的分析使用各种“纳米工具”，实现灵活和复杂的纳米尺度的刚度测量，粘附力测量，切割，和注射。E-SEM可以在纳米尺度上对生物样品进行实时观察，无需任何干燥或染色过程。如前所述，我们将该系统应用于生物标本的处理，如秀丽隐杆线虫（C。elegans）和酵母细胞。为了维持生物细胞的存活条件，重要的是减少纳米工具的交换时间。这对于在不破坏腔室压力的情况下使用各种纳米工具提高生物样本分析的效率也很重要。本文提出了一种新的纳米工具交换器系统，用于交换不同的纳米工具内的环境扫描电镜室。通过纳米工具交换器系统，主要获得以下优点，1）不需要打开样品室来交换纳米工具并再次排空样品室压力，2）不需要手动操作来交换纳米工具，3）可以通过交换新的纳米工具来回收纳米工具，4）可以连续使用不同的工具。首先，设计并制作出奈米工具转接器、奈米工具固定器及奈米工具保持器。最后，我们展示了基于E-SEM纳米操纵系统的不同纳米工具的交换。
A novel nanotool exchanger system is proposed based on Environmental Scanning Electron Microscope (E-SEM) nanorobotic manipulation system. We proposed to use the E-SEM nanomanipulation system for the analysis of biological specimen using various “nanotools” to realize flexible and complex nano-scale stiffness measurement, adhesion force measurement, cutting, and injection. The E-SEM can use to observe the biological samples in nano-scale and real-time without any drying or dyeing processes. As previous works, we applied the system to manipulate biological specimens, such as Caenorhabditis elegans (C. elegans) and yeast cells. To maintain the livable condition of biological cells, it is important to reduce the exchange time of the nanotools. This is also important to improve the efficiency of biological specimen analysis using various nanotools without break the chamber pressure. This paper presents a novel nanotool exchanger system for exchanging different nanotools within the ESEM chamber. Through the nanotool exchanger system, the following advantages are mainly obtained, 1) it is not needed to open the sample chamber to exchange the nanotools and to evacuate the sample chamber pressure again, 2) it is not needed to operate manually to exchange nanotools, 3) it is possible to recover the nanotools by exchanging new one, 4) it is possible to use different tools continuously. Firstly, the design and fabrication are presented for the proposed nanotool adaptor, nanotool attachment and nanotool holder. Finally, we demonstrate to exchange the different nanotools based on E-SEM nanomanipulation system.