First Isolates of Leptospira spp., from Rodents Captured in Angola.
First Isolates of Leptospira spp., from Rodents Captured in Angola.
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从安哥拉捕获的啮齿动物的第一个分离株属于钩端螺旋体。
DOI：
10.4269/ajtmh.15-0027
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发表时间：
2016-05-04
期刊：
影响因子：
--
通讯作者：
Vieira ML
中科院分区：
文献类型：
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作者：
Fortes-Gabriel E;Carreira T;Vieira ML
啮齿动物在致病性钩端螺旋体的传播中发挥着重要作用。然而，在安哥拉，既没有考虑这些螺旋体的天然宿主，也没有考虑钩端螺旋体病的诊断。关于这一差距，我们在罗安达和万博省捕获了啮齿动物，以识别传播的钩端螺旋体属。培养啮齿动物肾组织并扩增 DNA 并测序。用兔抗血清评估培养分离株的致病状态和分型；还进行了聚合酶链反应（PCR）和测序。共捕获啮齿类动物 37 只：家鼠（15 只，40.5%）、褐家鼠（9 只，24.3%）、小家鼠（13 只，35.2%）。 8 个 (21.6%) 肾脏样本中的钩端螺旋体 DNA 被扩增。从培养物中，我们获得了四种（10.8％）钩端螺旋体分离株，分别属于问号钩端螺旋体和博格彼得森钩端螺旋体基因种的黄疸出血和巴鲁姆血清群。这项研究提供了有关安哥拉大鼠和小鼠传播的循环钩端螺旋体的信息。
Rodents play an important role in the transmission of pathogenic Leptospira spp. However, in Angola, neither the natural reservoirs of these spirochetes nor leptospirosis diagnosis has been considered. Regarding this gap, we captured rodents in Luanda and Huambo provinces to identify circulating Leptospira spp. Rodent kidney tissue was cultured and DNA amplified and sequenced. Culture isolates were evaluated for pathogenic status and typing with rabbit antisera; polymerase chain reaction (PCR) and sequencing were also performed. A total of 37 rodents were captured: Rattus rattus (15, 40.5%), Rattus norvegicus (9, 24.3%), and Mus musculus (13, 35.2%). Leptospiral DNA was amplified in eight (21.6%) kidney samples. From the cultures, we obtained four (10.8%) Leptospira isolates belonging to the Icterohaemorrhagiae and Ballum serogroups of Leptospira interrogans and Leptospira borgpetersenii genospecies, respectively. This study provides information about circulating leptospires spread by rats and mice in Angola.