Decreased expression of the Id3 gene at 1p36.1 in ovarian adenocarcinomas.
Decreased expression of the Id3 gene at 1p36.1 in ovarian adenocarcinomas.
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卵巢腺癌中 Id3 基因 1p36.1 的表达降低。
DOI：
10.1054/bjoc.2000.1620
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发表时间：
2001-02-02
影响因子：
8.8
通讯作者：
Chenevix-Trench G
中科院分区：
文献类型：
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作者：
Arnold JM;Mok SC;Purdie D;Chenevix-Trench G
卵巢腺癌发生和发展的分子机制尚不明确。我们研究了基因表达的变化，卵巢癌细胞系相比，永生化的人卵巢表面上皮细胞系（HOSE）使用cDNA阵列。与HOSE细胞相比，我们在细胞系中鉴定了17个低表达基因和10个过表达基因。选择在卵巢癌细胞系中低表达的基因之一，Id 3，一种转录失活剂，用于进一步研究。与HOSE细胞相比，Id 3 mRNA在9个卵巢癌细胞系中的6个中以降低的水平表达，而在蛋白质水平上，所检查的所有7个卵巢癌细胞系以大大降低的水平表达Id 3蛋白。在原发性卵巢肿瘤中也检测到Id 3 mRNA的表达，仅在12/38（32%）例病例中发现。应用单链构象多态性（SSCP）分析法对原发性卵巢癌中Id 3基因的突变进行了研究。在94例卵巢肿瘤中，仅发现一个核苷酸替换，也存在于相应的宪法DNA中。1 p36位点洛缺失与Id 3表达缺失之间无相关性。这些数据表明Id 3不是1 p36处洛的靶标。© 2001年癌症研究运动http://www.bjcancer.com
The molecular events that drive the initiation and progression of ovarian adenocarcinoma are not well defined. We have investigated changes in gene expression in ovarian cancer cell lines compared to an immortalized human ovarian surface epithelial cell line (HOSE) using a cDNA array. We identified 17 genes that were under-expressed and 10 genes that were over-expressed in the cell lines compared to the HOSE cells. One of the genes under-expressed in the ovarian cancer cell lines, Id3, a transcriptional inactivator, was selected for further investigation. Id3 mRNA was expressed at reduced levels in 6 out of 9 ovarian cancer cell lines compared to the HOSE cells while at the protein level, all 7 ovarian cancer cell lines examined expressed the Id3 protein at greatly reduced levels. Expression of Id3 mRNA was also examined in primary ovarian tumours and was found in only 12/38 (32%) cases. A search was conducted for mutations of Id3 in primary ovarian cancers using single stranded conformation polymorphism (SSCP) analysis. Only one nucleotide substitution, present also in the corresponding constitutional DNA, was found in 94 ovarian tumours. Furthermore no association was found between LOH at 1p36 and lack of expression of Id3. These data suggest that Id3 is not the target of LOH at 1p36. © 2001 Cancer Research Campaign http://www.bjcancer.com