[The effects of cyclosporine A on immunoglobulins deposition in retina of streptozotocin-induced diabetic rats].
[The effects of cyclosporine A on immunoglobulins deposition in retina of streptozotocin-induced diabetic rats].
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发表时间：
2008-02
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通讯作者：
Xin Zhang;J. Cui;M. Qiu;De-qiang Li;P. Zhang;Jin-shi Zhang
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作者：
Xin Zhang;J. Cui;M. Qiu;De-qiang Li;P. Zhang;Jin-shi Zhang
目的研究糖尿病视网膜病变（DR）的自身免疫损伤及环孢素A（CsA）免疫抑制治疗对糖尿病大鼠DR的保护作用。方法将链脲佐菌素（STZ）诱导的糖尿病大鼠随机分为3组：糖尿病组（1组）、胰岛素治疗组（2组）和CsA治疗组（3组），CsA治疗组又分为2个亚组：A组：在高血糖出现前1周给予CsA，B组：在高血糖出现后1周给予CsA。A亚组和B亚组分别按CsA剂量（1、4和8 mg × kg（-1）× d（-1））分为3组。作为对照组，同时监测正常大鼠。HE染色观察视网膜病理改变，免疫组化和免疫荧光显微镜观察免疫球蛋白的沉积。结果8周后，糖尿病大鼠视网膜内IgG、伊加、IgM沉积明显。数据还表明，胰岛素治疗对DR没有影响。相反，CsA干预，糖尿病大鼠视网膜上的免疫球蛋白沉积消失。结论自身免疫损伤在糖尿病视网膜病变的发病机制中起重要作用。CsA免疫抑制治疗通过抑制免疫球蛋白在糖尿病大鼠视网膜中的沉积而显示出保护作用。
OBJECTIVE To study the autoimmune injuries on diabetic retinopathy (DR) and the protective effects of immunosuppressive therapy with cyclosporine A (CsA) on the DR of streptozotocin (STZ)-induced diabetic rats. METHODS STZ-induced diabetic rats were randomly divided into 3 groups: group 1: diabetic group without any treatment; group 2: insulin-treated group; group 3: CsA-treated group which was further divided into 2 subgroups: subgroup A: CsA was given 1 week before hyperglycemia appeared and subgroup B: CsA was given one week after hyperglycemia appeared. Subgroup A and subgroup B were further subdivided into 3 groups respectively, based on the dose of CsA (1, 4 and 8 mg x kg(-1) x d(-1)). As a control group, normal rats were also simultaneously monitored. The pathologic changes in the retina were investigated with HE stain and the deposition of immunoglobulins was detected with immunohistochemistry and immunofluorescent microscopy. RESULTS After 8 week, the deposition of IgG, IgA and IgM was quite significant in the retina of diabetic rats. The data also suggested that insulin treatment had no effects on the DR. In contrast,with CsA intervention, the deposition of immunoglobulins on the retina of diabetic rats vanished. CONCLUSIONS Autoimmune injuries were shown, in the present study, to play a critical role in the pathogenesis of diabetic retinopathy. Immunosuppressive treatment with CsA showed protective effects by inhibiting the deposition of immunoglobulins in the retina of diabetic rats.