Global quantitative SILAC phosphoproteomics reveals differential phosphorylation is widespread between the procyclic and bloodstream form lifecycle stages of Trypanosoma brucei.
Global quantitative SILAC phosphoproteomics reveals differential phosphorylation is widespread between the procyclic and bloodstream form lifecycle stages of Trypanosoma brucei.
复制标题
DOI：
10.1021/pr400086y
复制
发表时间：
2013-05-03
影响因子：
4.4
通讯作者：
Ferguson MA
中科院分区：
文献类型：
--
作者：
Urbaniak MD;Martin DM;Ferguson MA
我们报告了一个全球性的定量磷酸化蛋白质组学研究的血流和前循环形式的布氏锥虫使用SILAC标记的每个生命周期阶段。磷酸肽富集SCX和TiO 2导致识别的2551蛋白质组上的总共10096个磷酸化位点，并量化了两个生命周期阶段之间的8275个磷酸化位点的比率。其中超过9300个（92%）以前没有报告过。基于模型的基因富集分析确定了与鞭毛、蛋白激酶活性和基因表达调控相关的基因本体论术语的过度表达。定量数据显示，差异蛋白磷酸化是广泛的血液和前环形式的锥虫，具有显着的蛋白内差异磷酸化。尽管缺乏专用的酪氨酸激酶，但鉴定了234个磷酸酪氨酸残基，并且这些在两个生命周期阶段之间改变>10倍的位点中过度表示3-4倍。T.布鲁氏菌激酶组被磷酸化，有证据表明MAPK途径在两个生命周期阶段都有功能。T.布氏杆菌完全是转录后的，观察到的RNA结合蛋白的广泛磷酸化可能与该生物体中mRNA稳定性的控制有关。
We report a global quantitative phosphoproteomic study of bloodstream and procyclic form Trypanosoma brucei using SILAC labeling of each lifecycle stage. Phosphopeptide enrichment by SCX and TiO2 led to the identification of a total of 10096 phosphorylation sites on 2551 protein groups and quantified the ratios of 8275 phosphorylation sites between the two lifecycle stages. More than 9300 of these sites (92%) have not previously been reported. Model-based gene enrichment analysis identified over representation of Gene Ontology terms relating to the flagella, protein kinase activity, and the regulation of gene expression. The quantitative data reveal that differential protein phosphorylation is widespread between bloodstream and procyclic form trypanosomes, with significant intraprotein differential phosphorylation. Despite a lack of dedicated tyrosine kinases, 234 phosphotyrosine residues were identified, and these were 3–4 fold over-represented among site changing >10-fold between the two lifecycle stages. A significant proportion of the T. brucei kinome was phosphorylated, with evidence that MAPK pathways are functional in both lifecycle stages. Regulation of gene expression in T. brucei is exclusively post-transcriptional, and the extensive phosphorylation of RNA binding proteins observed may be relevant to the control of mRNA stability in this organism.
登录
查看更多内容
影响因子：
4.4
作者：
Cox, Juergen;Neuhauser, Nadin;Mann, Matthias
通讯作者：
Mann, Matthias
影响因子：
--
作者：
Pfister, Debora Domenicali;Burkard, Gabriela;Vassella, Erik
通讯作者：
Vassella, Erik
影响因子：
7
作者：
Savitski, Mikhail M.;Lemeer, Simone;Kuster, Bernhard
通讯作者：
Kuster, Bernhard
DOI：
10.1073/pnas.1210465109
发表时间：
2012-09-04
影响因子：
11.1
作者：
Barquilla, Antonio;Saldivia, Manuel;Navarro, Miguel
通讯作者：
Navarro, Miguel
三种致病性锥虫科：大型利什曼原虫、布氏锥虫和克氏锥虫的激酶组比较分析。
影响因子：
4.4
作者：
Parsons, M;Worthey, EA;Ward, PN;Mottram, JC
通讯作者：
Mottram, JC