Expression and function of mouse Sox17 gene in the specification of gallbladder/bile-duct progenitors during early foregut morphogenesis
Expression and function of mouse Sox17 gene in the specification of gallbladder/bile-duct progenitors during early foregut morphogenesis
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DOI：
10.1016/j.bbrc.2009.11.063
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发表时间：
2010-01-01
影响因子：
3.1
通讯作者：
Kanai, Yoshiakira
中科院分区：
文献类型：
--
作者：
Uemura, Mami;Hara, Kenshiro;Kanai, Yoshiakira
在早期器官发生阶段的小鼠胚胎中，靠近心管的前肠后腹侧内胚层形成肝脏。腹胰腺和胆囊肝和胰腺原基在体节早期出现在前肠腹侧内胚层的后段。然而，目前对胆囊原基和胆管原基的划分机制尚不清楚，我们证明了胆管和胆囊祖细胞在心脏区域外的成对外侧内胚层中指定的时间几乎与肝脏和胰腺在前确定内胚层诱导的时间相同，Sox17在前肠形成过程中发生在左侧和右侧前肠门(AIP)嘴唇后外侧最外侧区域内的特定人群中。在Sox17缺失的胚胎中，成对的Sox17阳性结构域向腹内侧扩展，合并在AIP唇部的中线，并定位于肝脏和胰腺原基之间，这些侧部结构域缺失。嵌合体分析显示，前肠后腹侧SOX17缺失的内胚层细胞不显示任何胆胆/胆管分子特征。我们的发现表明，Sox17在小鼠胚胎中自主地发挥细胞功能来指定胆囊管(C)2009 Elsevier Inc.保留所有权利
In early-organogenesis-stage mouse embryos, the posteroventral foregut endoderm adjacent to the heart tube gives rise to liver. ventral pancreas and gallbladder Hepatic and pancreatic primordia become specified in the posterior segment of the ventral foregut endoderm at early somite stages The mechanisms for demarcating gallbladder and bile duct primordium, however, are Poorly understood Here, we demonstrate that the gallbladder and bile duct progenitors are specified in the paired lateral endoderm domains outside the heart field at almost the same timing as hepatic and pancreatic induction In the anterior definitive endoderm, Sox17 reactivation occurs in a certain population within the most lateral domains posterolateral to the anterior intestinal portal (AIP) lip on both the left and right sides During foregut formation. the paired Sox17-positive domains expand ventromedially to merge in the midline of the AIP lip and become localized between the liver and pancreatic primordia In Sox17-null embryos, these lateral domains are missing. resulting in a complete loss of the gallbladder/bile-duct structure Chimera analyses revealed that Sox17-null endoderm cells in the posteroventral foregut do not display any gallbladder/bile-duct molecular characters. Our findings show that Sox17 functions cell-autonomously to specify gallbladder/bile-duct in the mouse embryo (C) 2009 Elsevier Inc All rights reserved