FMRFamide-immunoreactive retinopetal fibers in the frog, Rana pipiens: demonstration by lesion and immunocytochemical techniques.
FMRFamide-immunoreactive retinopetal fibers in the frog, Rana pipiens: demonstration by lesion and immunocytochemical techniques.
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青蛙 (Rana pipiens) 中的 FMRFamide 免疫反应性视网膜纤维：通过损伤和免疫细胞化学技术进行演示。
DOI：
10.1016/0006-8993(88)91030-x
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发表时间：
1988
期刊：
影响因子：
2.9
通讯作者：
Basinger,SF
中科院分区：
文献类型：
--
作者：
Wirsig-Wiechmann,CR;Basinger,SF
用免疫细胞化学和损伤技术研究了蛙视网膜纤维对软体动物心脏兴奋样肽（FMRFamide-ir）的免疫反应。在完整的青蛙，FMRFamide-ir视网膜纤维被发现在视神经，视神经乳头，神经纤维，神经节细胞和内丛状层的视网膜。假定单层无长突细胞也被标记。如在平卧视网膜中所观察到的，视网膜纤维从视盘向周边视网膜辐射，沿着其路线分支多次，并且在背侧视网膜中更普遍。压碎视神经后，除视神经大脑残端外，所有区域的视网膜纤维都消失了，这表明该投射来自中枢。双侧视交叉前病变完全消除retinopetal纤维从两个视网膜，表明纤维来自喙前脑。在吻侧脑内，在嗅球、斜角带、内侧隔、前连合区和后视前区的两个区域发现FMRFamide-ir胞体。嗅球切除术和中前脑病变同样减少了视网膜中这些纤维的数量，暗示终神经可能是一些视网膜投射的来源。这些数据将为进一步研究蛙视网膜中视网膜纤维的功能奠定基础。
Retinopetal fibers, immunoreactive to a molluscan cardioexcitatory-like peptide (FMRFamide-ir), were examined inRana pipienswith the use of immunocytochemical and lesion techniques. In intact frogs, FMRFamide-ir retinopetal fibers were found in the optic nerve, optic nerve head, and nerve fiber, ganglion cell and inner plexiform layers of the retina. Presumptive monostratified amacrine cells were also labeled. As observed in flat-mounted retinas, the retinopetal fibers radiated from the optic disc toward the peripheral retina, branched many times along their course and were more prevalent in the dorsal retina. Crushing the optic nerve eliminated retinopetal fibers from all regions except the cerebral stump of the optic nerve, indicating that this projection was of central origin. Bilateral prechiasmatic lesions completely eliminated retinopetal fibers from both retinas, indicating that the fibers arose from the rostral forebrain. Within the rostral brain, FMRFamide-ir perikarya were found in olfactory bulb, diagonal band, medial septum, anterior commissure area, and two regions of the posterior preoptic area. Olfactory bulbectomy and midforebrain lesions equally reduced the numners of these fibers in the retina, implicating the nervus terminalis as a possible source for some of the retinopetal projection. These data will serve as a foundation for future studies on the function of retinopetal fibers in the frog retina.