Polybrominated Diphenyl Ether-Associated Alterations in Cell Biochemistry as Determined by Attenuated Total Reflection Fourier-Transform Infrared Spectroscopy: a Comparison with DNA-Reactive and/or Endocrine-Disrupting Agents
Polybrominated Diphenyl Ether-Associated Alterations in Cell Biochemistry as Determined by Attenuated Total Reflection Fourier-Transform Infrared Spectroscopy: a Comparison with DNA-Reactive and/or Endocrine-Disrupting Agents
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DOI：
10.1021/es8036127
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发表时间：
2009-05-01
影响因子：
11.4
通讯作者：
Martin, Francis L.
中科院分区：
文献类型：
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作者：
Llabjani, Valon;Jones, Kevin C.;Martin, Francis L.
鉴于多溴联苯醚的环境负担不断增加，多溴联苯醚是否会对靶细胞产生影响变得越来越重要。衰减全反射傅里叶变换红外（ATR-FTIR）光谱可用于基于衍生出红外（IR）光谱形式的详细“生化细胞指纹”的概念来生化标记细胞。通过采用随后的计算方法，例如主成分分析（PCA）和/或线性判别分析（LDA），实现数据简化以允许鉴定波数相关的生物标志物的效果。相似光谱（或分数）远离不相似光谱的聚类突出了负责区分类别的方差。区别性生物标志物可能包括蛋白质构象变化、DNA/RNA的结构改变、糖原含量或蛋白质磷酸化。采用这种方法，我们在MCF-7细胞中研究了多溴二苯醚（同系物47、153、183和209）、苯并[a]芘（B[a]P）、2-氨基-1-甲基-6-苯基咪唑[4，5-B]吡啶（PHIP）、17 β-雌二醇（E-2）或林丹（γ-六氯环己烷）的剂量相关效应。将浓缩在G（0）/G（1）-或S-期的培养物处理24小时。处理后，将MCF-7细胞固定并应用于IR反射Low-E窗口，以使用ATR-FTIR光谱进行询问。在培养物中低至10（-12）M的浓度下，
Whether polybrominated diphenyl ethers (PBDEs) induce effects in target cells is increasingly important given that their environmental burdens are rising. Attenuated total reflection Fourier-transform infrared (ATR-FTIR) spectroscopy can be used to biochemically signature cells based on the notion that a detailed "biochemical-cell fingerprint" in the form of an infrared (IR) spectrum is derived. By employing subsequent computational approaches such as principal component analysis (PCA) and/or linear discriminant analysis (LDA), data reduction is achieved to allow for the identification of wavenumber-related biomarkers of effect. Clustering of similar spectra (or scores) away from dissimilar ones highlights the variance responsible for discriminating classes. Discriminating biomarkers might include protein conformational changes, structural alterations to DNA/RNA, glycogen content, or protein phosphorylation. Employing this approach,we investigated in MCF-7 cells the dose-related effects of PBDEs (congeners 47, 153, 183, and 209), benzo[a]pyrene (B[a]P), 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PHIP), 17 beta-Oestradiol (E-2) or lindane (gamma-hexachlorocyclohexane). Cultures concentrated in G(0)/G(1)- or S-phases were treated for 24 h. Following treatment, MCF-7 cells were fixed and applied to IR reflective Low-E windows for interrogation using ATR-FTIR spectroscopy. At concentrations as low as 10(-12) M in culture, significant separation (P