Analysis of RNA-Seq, DNA Target Enrichment, and Sanger Nucleotide Sequence Data Resolves Deep Splits in the Phylogeny of Cuckoo Wasps (Hymenoptera: Chrysididae)
Analysis of RNA-Seq, DNA Target Enrichment, and Sanger Nucleotide Sequence Data Resolves Deep Splits in the Phylogeny of Cuckoo Wasps (Hymenoptera: Chrysididae)
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DOI：
10.1093/isd/ixaa018
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发表时间：
2021-01
影响因子：
3.4
通讯作者：
Thomas Pauli;K. Meusemann;Sandra Kukowka;Manuela Sann;A. Donath;C. Mayer;J. Oeyen;Yolanda Ballesteros;Alexander Berg;E. V. Berghe;Hermes E Escalona;A. Guglielmino;M. Niehuis;M. Olmi;L. Podsiadlowski;C. Polidori;Jeroen de Rond;P. Rosa;T. Schmitt;F. Strumia;Mareike Wurdack;Shanlin Liu;Xin Zhou;B. Misof;R. Peters;O. Niehuis
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作者：
Thomas Pauli;K. Meusemann;Sandra Kukowka;Manuela Sann;A. Donath;C. Mayer;J. Oeyen;Yolanda Ballesteros;Alexander Berg;E. V. Berghe;Hermes E Escalona;A. Guglielmino;M. Niehuis;M. Olmi;L. Podsiadlowski;C. Polidori;Jeroen de Rond;P. Rosa;T. Schmitt;F. Strumia;Mareike Wurdack;Shanlin Liu;Xin Zhou;B. Misof;R. Peters;O. Niehuis
摘要黄蜂科(杜鹃黄蜂、金黄蜂)仅由寄生类和盗寄生种类组成，其中许多种类具有令人惊叹的虹彩色彩和对其寄生生活方式的表型适应。以前基于Sanger序列数据推断该科主要谱系(亚科、部落、属)之间的系统发育关系的尝试不足以在统计上解析Amiseginae亚科的单系和系统发育地位，以及Alallcoeliini、Chrysidini、Elampini和Parnopini(叶甲亚科)之间的系统发育关系。在这里，我们通过结合RNA-seq和DNA靶标浓缩数据，从94种叶甲科(代表蝶科、叶蜂科、鳞甲科、叶蜂科)和45种外群物种的492个核单拷贝基因(230,915个氨基酸位点)的核苷酸序列数据中推断出系统发育。我们发现对Amiseginae的支持与Ceptinae的亲缘关系比与Crysidinae的亲缘关系更密切。此外，我们发现强烈支持阿罗科里尼是所有剩余的金龟亚科的姐妹血统，而伊兰皮尼代表金龟子和帕诺皮尼的姐妹血统。我们的研究证实了最近的一项系统基因组研究的结果，该研究揭示了菊科可能是副反应性的。图形摘要
Abstract The wasp family Chrysididae (cuckoo wasps, gold wasps) comprises exclusively parasitoid and kleptoparasitic species, many of which feature a stunning iridescent coloration and phenotypic adaptations to their parasitic life style. Previous attempts to infer phylogenetic relationships among the family's major lineages (subfamilies, tribes, genera) based on Sanger sequence data were insufficient to statistically resolve the monophyly and the phylogenetic position of the subfamily Amiseginae and the phylogenetic relationships among the tribes Allocoeliini, Chrysidini, Elampini, and Parnopini (Chrysidinae). Here, we present a phylogeny inferred from nucleotide sequence data of 492 nuclear single-copy genes (230,915 aligned amino acid sites) from 94 species of Chrysidoidea (representing Bethylidae, Chrysididae, Dryinidae, Plumariidae) and 45 outgroup species by combining RNA-seq and DNA target enrichment data. We find support for Amiseginae being more closely related to Cleptinae than to Chrysidinae. Furthermore, we find strong support for Allocoeliini being the sister lineage of all remaining Chrysidinae, whereas Elampini represent the sister lineage of Chrysidini and Parnopini. Our study corroborates results from a recent phylogenomic investigation, which revealed Chrysidoidea as likely paraphyletic. Graphical Abstract