Colorimetric Detection of Copper(II) Ion Using Click Chemistry and Hemin/G-Quadruplex Horseradish Peroxidase-Mimicking DNAzyme
Colorimetric Detection of Copper(II) Ion Using Click Chemistry and Hemin/G-Quadruplex Horseradish Peroxidase-Mimicking DNAzyme
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DOI：
10.1021/ac501739a
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发表时间：
2014-07-01
影响因子：
7.4
通讯作者：
Zeng, Lingwen
中科院分区：
文献类型：
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作者：
Ge, Chenchen;Luo, Quan;Zeng, Lingwen
G-四链体形成序列可以通过铜（I）离子（Cu+）催化的点击化学反应在水溶液中的叠氮化物和炔修饰的富G短序列之间形成，消除了常规测定的固定和洗涤步骤。Cu+的来源是由抗坏血酸钠还原Cu 2+产生的。在氯化血红素和K+存在下，氯化血红素/G-四链体结构的自组装具有辣根过氧化物酶（HRP）的活性，能催化其无色底物四甲基联苯胺（TMB）生成有色产物。因此，Cu 2+的浓度可以根据溶液的颜色变化进行目视评价以用于定性分析，并且还使用酶标仪记录所得溶液在450 nm处的光密度（OD）值以用于定量分析。
G-quadruplex-forming sequence can be formed through a copper(I) ion (Cu+)-catalyzed click chemistry between azide- and alkyne-modified short G-rich sequences in aqueous solution, eliminating immobilization and washing steps of conventional assays. The source for Cu+ was generated from the reduction of Cu2+ with the reductant of sodium ascorbate. In the presence of hemin and K+, the self-assembly of hemin/G-quadruplex structure has the activity of horseradish peroxidase (HRP), which can catalyze its colorless substrate tetrazmethyl benzidine (TMB) into a colored product. Hence, the concentration of Cu2+ can be evaluated visually for qualitative analysis according to the color change of the solution, and the optical density (OD) value of the resulting solution at 450 nm was also recorded using a microplate reader for quantitative analysis.