Differential miRNA expression profiles between the first and third trimester human placentas
Differential miRNA expression profiles between the first and third trimester human placentas
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DOI：
10.1152/ajpendo.00660.2012
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发表时间：
2013-04-01
影响因子：
5.1
通讯作者：
Wang, Yuping
中科院分区：
文献类型：
--
作者：
Gu, Yang;Sun, Jingxia;Wang, Yuping
顾莹，孙洁，Groome LJ，王莹。妊娠早期和晚期胎盘miRNA表达谱的差异分析。[J] .中国生物医学工程学报，2013,31(4):836- 843。首次发表于2013年2月26日；doi: 10.1152 / ajpendo.00660.2012。为了测定不同胎龄胎盘microRNA （miRNA）的表达，我们从6个妊娠早期和晚期胎盘中分离总RNA。通过Affymetrix miRNA微阵列分析miRNA表达，通过mircluster和miRGen Cluster网络程序鉴定miRNA簇。采用qRT-PCR验证miRNA表达，采用原位杂交（ISH）确定绒毛组织中miRNA的区室定位。共有208个miRNA转录本，代表191个成熟miRNA，在妊娠早期和晚期胎盘中表达不同。miR-17-92集群、C14MC、miR-371集群和C19MC中的mirna在妊娠早期胎盘中显著上调。相比之下，let-7家族、miR-34家族、miR-29a集群、miR-195集群和miR-181c集群的mirna在妊娠晚期胎盘中显著上调。qRT-PCR证实妊娠早期胎盘miR-371-5p、miR-17-3p和miR-708-5p表达升高，miR-125b-5p和miR-139-5p表达降低。ISH证实了miR-371-5p和miR-125b-5p在绒毛组织中的不同表达模式。在妊娠早期和晚期胎盘中发现了不同的miRNA簇表达谱。调节先天/适应性免疫反应的mirna在妊娠早期和晚期胎盘中均有强烈表达。发挥致癌、血管生成和抗凋亡特性的mirna主要在妊娠早期胎盘中表达，而促进细胞分化和肿瘤抑制功能的mirna在妊娠晚期胎盘中强烈表达。这些结果表明，mirna在胎盘发育中起着关键作用。
Gu Y, Sun J, Groome LJ, Wang Y. Differential miRNA expression profiles between the first and third trimester human placentas. Am J Physiol Endocrinol Metab 304: E836-E843, 2013. First published February 26, 2013; doi:10.1152/ajpendo.00660.2012.-To determine placental microRNA (miRNA) expression at different gestational age, total RNA from six first and six third trimester placentas was isolated. miRNA expression was analyzed by Affymetrix miRNA microarray, and miRNA clusters were identified by web-based programs MirClust and miRGen Cluster. qRT-PCR was carried out to validate miRNA expression, and in situ hybridization (ISH) was performed to determine compartmental localization of miRNAs within villous tissue. A total of 208 miRNA transcripts, which represent 191 mature miRNAs, were found differently expressed between first and third trimester placentas. miRNAs within the miR-17-92 cluster, C14MC, miR-371 cluster, and C19MC were significantly upregulated in the first trimester placentas. In contrast, miRNAs of the let-7 family, miR-34 family, miR-29a cluster, miR-195 cluster, and miR-181c cluster were significantly upregulated in the third trimester placentas. Increased miR-371-5p, miR-17-3p, and miR-708-5p expression and decreased miR-125b-5p and miR-139-5p expression in the first trimester placentas were confirmed by qRT-PCR. Different expression pattern for miR-371-5p and miR-125b-5p within villous tissue was demonstrated by ISH. Distinct miRNA cluster expression profiles between the first and third trimester placentas were identified. miRNAs that regulate innate/adaptive immune responses are strongly expressed in both first and third trimester placentas. miRNAs that exert oncogenic, angiogenic, and antiapoptotic properties are dominantly expressed in the first trimester placentas, whereas miRNAs that promote cell differentiation and function as tumor suppressors are strongly expressed in the third trimester placentas. These results indicate that miRNAs play critical roles in placental development.