Genome-wide identification and expression profile analysis of the HOG gene family in Aspergillus oryzae
Genome-wide identification and expression profile analysis of the HOG gene family in Aspergillus oryzae
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米曲霉 HOG 基因家族的全基因组鉴定和表达谱分析。
DOI：
10.1007/s11274-018-2419-6
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发表时间：
2018-02-01
影响因子：
4.1
通讯作者：
Zeng, Bin
中科院分区：
文献类型：
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作者：
He, Bin;Tu, Yayi;Zeng, Bin
高渗透压甘油(HOG)基因家族在真菌的多种发育和生理过程中发挥着重要作用，如细胞膜的通透性、厚壁孢子的形成和胁迫信号等。虽然在酿酒酵母和一些丝状真菌中已经对HOG基因的功能进行了研究，但在主要用于酱油生产的真菌米曲霉中还没有对HOG基因家族进行全面的分析。在这项研究中，我们从米曲霉基因组中鉴定并纠正了90个猪基因。根据系统发育关系，将它们分为4个独立的类群(A-D组)，分别由16、24、30和20个蛋白质组成。分析了所有HOG蛋白的6个保守基序和外显子-内含子结构，揭示了AoHOG基因的多样性。基于转录组技术，鉴定了AoHOG基因在各发育阶段的表达模式，表明AoHOG基因家族主要在对数发育阶段发挥作用。AoHOG基因在不同浓度盐胁迫下的表达谱表明，AoHOG基因广泛参与盐胁迫响应，可能存在不同的机制。AoHOG基因的全基因组鉴定、进化、基因结构和表达分析为该基因家族及其在发育和胁迫反应中的潜在参与提供了一个全面的概述。本研究结果为深入研究猪基因家族的生理生化功能奠定了基础。
The High osmolarity glycerol (HOG) gene family plays crucial roles in various developmental and physiological processes in fungi, such as the permeability of cell membrane, chlamydospore formation and stress signaling. Although the function of HOG genes has been investigated in Saccharomyces cerevisiae and some filamentous fungi, a comprehensive analysis of HOG gene family has not been performed in Aspergillus oryzae, a fungi mainly used for the production of soy sauce. In this study, we identified and corrected a total of 90 HOG genes from the A. oryzae genome. According to the phylogenetic relationship, they were divided into four discrete groups (Group A-D) comprising of 16, 24, 30 and 20 proteins, respectively. Six conserved motifs and exon-intron structures were examined among all HOG proteins to reveal the diversity of AoHOG genes. Based on transcriptome technology, the expression patterns of AoHOG genes across all developmental stages was identified, suggesting that the AoHOG gene family mainly functions in the logarithmic phase of development. The expression profiles of AoHOG genes under different concentrations of salt stress indicated that AoHOG genes are extensively involved in salt stress response, with possibly different mechanisms. The genome-wide identification, evolutionary, gene structures and expression analyses of AoHOG genes provide a comprehensive overview of this gene family as well as their potential involvements in development and stress responses. Our results will facilitate further research on HOG gene family regarding their physiological and biochemical functions.