Development and characterization of a standardized ELISA including a reference serum on each plate to detect antibodies induced by experimental malaria vaccines
Development and characterization of a standardized ELISA including a reference serum on each plate to detect antibodies induced by experimental malaria vaccines
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DOI：
10.1016/j.vaccine.2007.10.064
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发表时间：
2008-01-10
期刊：
影响因子：
5.5
通讯作者：
Long, Carole A.
中科院分区：
文献类型：
--
作者：
Miura, Kazutoyo;Orcutt, Andrew C.;Long, Carole A.
酶联免疫吸附试验(EL ISA)已被广泛用于检测抗体滴度，以评价疫苗的免疫原性。然而，在实验疫苗的情况下，由于通常没有统一的标准，目前还没有普遍接受的表达ELISA结果的方法。终点法和单一稀释法都有明显的缺点。在本文中，我们通过在每个酶标板上加入连续稀释的标准血清，以较少的样品稀释度获得了重复性较好的数据。由于与其他方法相比，该方法可以提供可靠的抗体滴度，因此可以有力地支持疫苗的开发。爱思唯尔有限公司出版。
Enzyme linked immunosorbent assay (ELISA) has been widely used to measure antibody titers for evaluating the immunogenicity of a vaccine. However, there is as yet no generally accepted way of expressing the ELISA results in the case of experimental vaccines, since there is usually no uniform standard. Both end point and single dilution methods have significant disadvantages. In this paper, we obtained reproducible data with fewer dilutions of samples by addition of serially diluted standard serum to each ELISA plate. Since this ELISA method gives reliable antibody titer with less labor than other methods, it can strongly support vaccine development. Published by Elsevier Ltd.