An Optimized Method for Delivering Flow Tracer Particles to Intravital Fluid Environments in the Developing Zebrafish
An Optimized Method for Delivering Flow Tracer Particles to Intravital Fluid Environments in the Developing Zebrafish
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DOI：
10.1089/zeb.2012.0740
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发表时间：
2012-09-01
期刊：
影响因子：
2
通讯作者：
Hove, Jay R.
中科院分区：
文献类型：
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作者：
Craig, Michael P.;Gilday, Steven D.;Hove, Jay R.
越来越多的证据表明，活体内流动-结构相互作用是正常胚胎发育和疾病进展的关键形态来源，但旨在研究这些相互作用的流体力学研究在可视化和量化流体流动的能力方面一直受到限制。在这项研究中，我们描述了一种注射小分子斑马鱼的方案(每条鱼=50000示踪剂)是常规实现的，并且对斑马鱼的生理或长期生存没有不利影响。本文报道的数据和方法将被证明对广泛的活体成像技术[例如，粒子跟踪测速、MU-多普勒、数字粒子图像测速(DPIV)和四维DPIV]将被证明是有价值的，这些技术依靠示踪粒子来可视化和量化斑马鱼发育中的流体流动。
Growing evidence suggests that intravital flow-structure interactions are critical morphogens for normal embryonic development and disease progression, but fluid mechanical studies aimed at investigating these interactions have been limited in their ability to visualize and quantify fluid flow. In this study, we describe a protocol for injecting small (= 50 thousand tracers per fish) were routinely achieved and had no adverse effects on zebrafish physiology or long-term survivorship. The data and methods reported here will prove valuable for a broad range of in vivo imaging technologies [e. g., particle-tracking velocimetry, mu-Doppler, digital particle image velocimetry (DPIV), and 4-dimensional-DPIV] which rely on tracer particles to visualize and quantify fluid flow in the developing zebrafish.