Characteristic and evolution of HAT and HDAC genes in Gramineae genomes and their expression analysis under diverse stress in Oryza sativa
Characteristic and evolution of HAT and HDAC genes in Gramineae genomes and their expression analysis under diverse stress in Oryza sativa
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禾本科基因组中HAT和HDAC基因的特征、进化及其在不同胁迫下的表达分析
DOI：
10.1007/s00425-021-03589-1
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发表时间：
2021-03-01
期刊：
影响因子：
4.3
通讯作者：
Li, Lijia
中科院分区：
文献类型：
--
作者：
Hou, Jiaqi;Ren, Ruifei;Li, Lijia
禾本科 HAT 和 HDAC 的综合表征揭示了它们的保守性和变异性。 CBP 和 RPD/HDA1 基因家族中最近的 WGD/SD 基因对可能赋予特定的适应性进化变化。 OsHAT 和 OsHDAC 基因的表达为发育的不同方面和对不同压力的反应提供了新的视野。组蛋白乙酰化酶 (HAT) 和组蛋白脱乙酰化酶 (HDAC) 已被证明紧密相连，通过调节组蛋白乙酰化水平在植物生长、发育和对非生物胁迫的响应中发挥关键作用。然而，禾本科中 HAT 和 HDAC 的进化动力学和功能分化仍不清楚。在本研究中，我们通过详细分析，在7个禾本科基因组中鉴定出了37个HAT基因和110个HDAC基因。构建这些 HAT 和 HDAC 蛋白的系统发育树是为了说明禾本科植物中的进化关系。基因结构、蛋白质特性和蛋白质基序组成说明了禾本科植物中 HAT 和 HDAC 的保守性和变异性。基因重复分析表明，最近的全基因组重复（WGD）/片段重复（SD）事件导致了禾本科中CBP和RPD3/HDA1基因家族的多样化。此外，启动子顺式元件预测表明OsHATs和OsHDACs可能是功能蛋白并参与多种信号通路。 RNA-seq数据的表达分析表明，所有OsHAT和OsHDAC基因在不同组织或发育阶段表达，表明它们普遍表达。此外，我们发现它们的表达模式因寒冷、干旱、盐、光、脱落酸（ABA）和吲哚-3-乙酸（IAA）处理而改变。这些发现为进一步鉴定候选OsHAT和OsHDAC基因提供了基础，这些基因可用于调节生长发育和提高作物对非生物胁迫的耐受性。
Comprehensive characterization of Gramineae HATs and HDACs reveals their conservation and variation. The recent WGD/SD gene pairs in the CBP and RPD/HDA1 gene family may confer specific adaptive evolutionary changes. Expression of OsHAT and OsHDAC genes provides a new vision in different aspects of development and response to diverse stress. The histone acetylase (HAT) and histone deacetylase (HDAC) have been proven to be tightly linked to play a crucial role in plant growth, development and response to abiotic stress by regulating histone acetylation levels. However, the evolutionary dynamics and functional differentiation of HATs and HDACs in Gramineae remain largely unclear. In the present study, we identified 37 HAT genes and 110 HDAC genes in seven Gramineae genomes by a detailed analysis. Phylogenetic trees of these HAT and HDAC proteins were constructed to illustrate evolutionary relationship in Gramineae. Gene structure, protein property and protein motif composition illustrated the conservation and variation of HATs and HDACs in Gramineae. Gene duplication analysis suggested that recent whole genome duplication (WGD)/segmental duplication (SD) events contributed to the diversification of the CBP and RPD3/HDA1 gene family in Gramineae. Furthermore, promoter cis-element prediction indicated that OsHATs and OsHDACs were likely functional proteins and involved in various signaling pathways. Expression analysis by RNA-seq data showed that all OsHAT and OsHDAC genes were expressed in different tissues or development stages, revealing that they were ubiquitously expressed. In addition, we found that their expression patterns were altered in response to cold, drought, salt, light, abscisic acid (ABA), and indole-3-acetic acid (IAA) treatments. These findings provide the basis for further identification of candidate OsHAT and OsHDAC genes that may be utilized in regulating growth and development and improving crop tolerance to abiotic stress.