Impaired endothelium-dependent vasodilation in type 9 diabetes -: Relation to LDL size, oxidized LDL, and antioxidants
Impaired endothelium-dependent vasodilation in type 9 diabetes -: Relation to LDL size, oxidized LDL, and antioxidants
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DOI：
10.2337/diacare.22.6.973
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发表时间：
1999-06-01
期刊：
影响因子：
16.2
通讯作者：
Yki-Järvinen, H
中科院分区：
文献类型：
--
作者：
Mäkimattila, S;Liu, ML;Yki-Järvinen, H
目的-寻找2型糖尿病内皮功能障碍的决定因素。研究设计和方法-我们对心血管风险标志物进行了全面分析，并测量了对内皮依赖性的血流反应（乙酰胆碱[ACh]和N-G-单甲基-L-精氨酸）和非依赖性硝普钠[SNP]血管活性药物对30例非吸烟男性2型糖尿病患者的影响（年龄51 +/- 1岁，BMI 27.8 +/- 0.4 kg/m（2），HbA（1c）7.4 +/- 0.3%）和12名匹配的正常对照男性。（6.1 ± 0.5）比正常受试者（9.7 ± 1.5 ml.dl（-1）.min（-1），P < 0.01），而SNP期间的流量相似（9.1 ± 0.6 vs. 9.9 ± 1.3 ml.dl（-1）.min（-1），NS）。2型糖尿病患者的内皮依赖性与非内皮依赖性血流的比值（ACh：SNP比值）（0.70 ± 0.05）比正常人（1.10 ± 0.18，P < 0.01）低31%。2型糖尿病患者的总甘油三酯（2.2 ± 0.4 mmol/l vs. 1.3 ± 0.2 mmol/l，P < 0.05）、极低密度脂蛋白（VLDL）和中密度脂蛋白甘油三酯显著高于正常人，平均LDL颗粒直径显著小于正常人。在2型糖尿病患者（183 +/- 7）和正常受试者（183 +/- 9 min，NS）中，Cu 2+体外氧化LDL的滞后时间相似。测量和计算（血清中单个抗氧化剂的浓度总和）总过氧化氢自由基捕获能力（TRAPs）组之间是相当的。在2型糖尿病患者中，LDL大小与内皮依赖性血管舒张功能（r = 0.43，P < 0.05）、血清甘油三酯（r =-0.75，P < 0.001）和LDL体外氧化滞后时间（r = 0.38，P < 0.05）显着相关。HbA（1c）tvas与体外LDL氧化滞后时间呈负相关（r =-0.41，P < 0.05），TRAP也呈负相关。结论：总之，2型糖尿病患者体内内皮依赖性血管舒张功能受损，血清甘油三酯升高，LDL大小减少，抗氧化能力正常。在这些参数中，LDL大小与内皮功能显著相关。
OBJECTIVE - To search for determinants of endothelial dysfunction in type 2 diabetes.RESEARCH DESIGN AND METHODS - We performed a comprehensive analysis of cardiovascular risk markers and measured blood flow responses to endothelium-dependent (acetylcholine [ACh] and N-G-monomethyl-L-arginine) and -independent (sodium nitroprusside [SNP]) vasoactive agents in 30 nonsmoking men with type 2 diabetes (age 51 +/- 1 years, BMI 27.8 +/- 0.4 kg/m(2), HbA(1c) 7.4 +/- 0.3%) and 12 matched normal control men.RESULTS - ACh-induced vasodilation was 37% lower in type 2 diabetic (6.1 + 0.5) than in normal subjects (9.7 +/- 1.5 ml.dl(-1).min(-1), P < 0.01), while flows during SNP were similar (9.1 +/- 0.6 vs. 9.9 +/- 1.3 ml.dl(-1).min(-1), NS). The ratio of endothelium-dependent vs. -independent flow (ACh:SNP ratio) was 31% lower in type 2 diabetic (0.70 +/- 0.05) than in normal subjects (1.10 +/- 0.18, P < 0.01). Total (2.2 +/- 0.4 vs. 1.3 +/- 0.2 mmol/l, P < 0.05), VLDL, and intermediate-density lipoprotein triglycerides were significantly higher, and the mean LDL particle diameter was significantly smaller in type 2 diabetic than in normal subjects. The lag times for LDL oxidation by Cu2+ in vitro were similar in patients with type 2 diabetes (183 +/- 7) and in normal subjects (183 +/- 9 min, NS). Measured and calculated (sum of concentration of individual antioxidants in serum) total peroxyl radical-trapping capacities (TRAPs) were comparable between the groups. In the patients with type 2 diabetes, LDL size was significantly correlated with endothelium-dependent vasodilation (r = 0.43, P < 0.05), serum triglycerides (r = -0.75, P < 0.001), and the lag time for LDL oxidation in vitro (r = 0.38, P < 0.05). HbA(1c) tvas inversely correlated with the lag time for LDL oxidation in vitro (r = -0.41,P < 0.05) and TRAPCONCLUSIONS - In summary, patients with type 2 diabetes exhibited impaired endothelium-dependent vasodilation in vivo, elevated serum triglycerides, decreased LDL size, and normal antioxidant capacity. Of these parameters, LDL size was significantly correlated with endothelial function.