Regional differences in glycoconjugates of intestinal M cells in mice: potential targets for mucosal vaccines.
Regional differences in glycoconjugates of intestinal M cells in mice: potential targets for mucosal vaccines.
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DOI：
10.1152/ajpgi.1994.267.6.g1108
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发表时间：
1994-12
期刊：
影响因子：
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通讯作者：
Paul J. Giannasca;K. T. Giannasca;P. Falk;J. Gordon;Marian R. Neutra
中科院分区：
文献类型：
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作者：
Paul J. Giannasca;K. T. Giannasca;P. Falk;J. Gordon;Marian R. Neutra
我们使用一组凝集素和抗体来描述与成年BALB/c小鼠肠道不同区域的M细胞相关的复杂碳水化合物的组成。岩藻糖特异性凝集素Ulex uropaeus凝集素I(UEA I)是小肠M细胞的标志物，可在分化早期识别M细胞。不同的岩藻糖特异性探针可以区分单个滤泡相关上皮(FAE)中的M细胞亚群。某些凝集素显示，M细胞具有延伸到底层淋巴组织的基本突起。结肠和直肠的M细胞表现出不同于Peyer‘s斑块的M细胞的糖基化模式，并以末端半乳糖为特征。在体内，UEA I选择性地黏附在粘膜外植体和结扎肠环中的Peyer‘s Patch M细胞上。凝集素被摄取到内吞小泡中，并运输到上皮内袋和基底外侧膜的其他区域。因此，M细胞特异性的糖结合物可以作为“受体”，将凝集素-抗原结合物靶向于粘膜免疫系统。
We have used a panel of lectins and antibodies to describe the composition of complex carbohydrates associated with M cells in various regions of the intestinal tract of adult BALB/c mice. The fucose-specific lectin Ulex europaeus agglutinin type I (UEA I) is a marker of M cells in the small intestine and recognized M cells at an early stage of differentiation. Subpopulations of M cells in a single follicle-associated epithelium (FAE) could be distinguished by different fucose-specific probes. Certain lectins revealed that M cells have basal processes that extend into the underlying lymphoid tissue. Colonic and rectal M cells display glycosylation patterns distinct from M cells of Peyer's patches and are characterized by terminal galactose. UEA I selectively adhered to Peyer's patch M cells in mucosal explants and in ligated intestinal loops in vivo. The lectin was taken up into endocytic vesicles and transported to the intra-epithelial pocket and other domains of the basolateral membrane. Thus M cell-specific glycoconjugates could serve as "receptors" for targeting of lectin-antigen conjugates to the mucosal immune system.