Population structure and characterisation of Staphylococcus aureus from bacteraemia at multiple hospitals in China: association between antimicrobial resistance, toxin genes and genotypes
Population structure and characterisation of Staphylococcus aureus from bacteraemia at multiple hospitals in China: association between antimicrobial resistance, toxin genes and genotypes
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中国多家医院菌血症金黄色葡萄球菌的种群结构和特征：抗菌药物耐药性、毒素基因和基因型之间的关联
DOI：
10.1016/j.ijantimicag.2013.04.031
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发表时间：
2013-09-01
影响因子：
10.8
通讯作者：
Wang, Hui
中科院分区：
文献类型：
--
作者：
He, Wenqiang;Chen, Hongbin;Wang, Hui
对中国多家医院的菌血症金黄色葡萄球菌进行了基因鉴定，以提高对其流行病学的认识。在中国16家医院共收集到236株连续、非重复的金黄色葡萄球菌菌血症分离株。菌株经抗菌药物耐药性鉴定、19个毒素基因鉴定、agr等位基因鉴定、多位点序列分型和spa分型鉴定。耐甲氧西林金黄色葡萄球菌（MRSA）感染率为47.5%（112/236）。共鉴定出42种序列类型（STs）和63种spa类型，其中MRSA为14种STs和14种spa类型。克隆复合体（CC） 8、CC5、ST7和CC188占67.4%。MRSA主要基因型为ST239-t030/t037- sccmecii - agri(50%)，其次为ST5-t002/t570-SCCmecII-agrII（8%）。ST5-SCCmecII和ST239-t037-SCCmecIII中万古霉素MIC = >= 1 mg/L的检出率显著高于ST239-t030-SCCmecIII，而利福平耐药与ST239-t030-SCCmecIII的检出率压倒性相关（P < 0.001）。Oxacillin对ST59-MRSA的mic相对较低。金黄色葡萄球菌（MSSA）主要基因型为ST7-t091/t796-agrI（16.1%）、ST188-t189-agrI（12.1%）和ST398-t571/t034-agrI（5.6%）。毒素基因检出率为95.8%，共形成89条毒素基因谱。毒素基因sea、selk、selq和sell在MRSA中更为常见，而tsst-1、seb、sed、selm、seln、selp和selj在MSSA中更为常见（P < 0.001）。pvl基因在CC59中更为常见，而tsst-1基因在CC15、CC188和ST398中更为常见（P < 0.001）。主要基因型与特定的抗菌素耐药性和毒素基因谱相关。(C) 2013 Elsevier b.v.与International Society of Chemotherapy。版权所有。
Staphylococcus aureus from bacteraemia at multiple hospitals in China were genetically characterised to improve understanding of its epidemiology. A total of 236 consecutive, non-duplicate S. aureus bacteraemia isolates were collected at 16 Chinese hospitals. Isolates were characterised by antimicrobial resistance, 19 toxin genes, agr alleles, multilocus sequence typing and spa typing. The prevalence of meticillin-resistant S. aureus (MRSA) was 47.5% (112/236). Forty-two sequence types (STs) and 63 spa types were identified, including 14 STs and 14 spa types for MRSA. Clonal complex (CC) 8, CC5, ST7 and CC188 accounted for 67.4% of the isolates. ST239-t030/t037-SCCmecIII-agrI was the predominant MRSA genotype (50%), followed by ST5-t002/t570-SCCmecII-agrII (8%). A vancomycin MIC = >= 1 mg/L was detected significantly more often in ST5-SCCmecII and ST239-t037-SCCmecIII, whereas rifampicin resistance was overwhelmingly associated with ST239-t030-SCCmecIII (P < 0.001). Oxacillin MICs were relatively low for ST59-MRSA. Major genotypes of meticillin-susceptible S. aureus (MSSA) were ST7-t091/t796-agrI (16.1%), ST188-t189-agrI (12.1%) and ST398-t571/t034-agrI (5.6%). Toxin genes were identified in 95.8% of isolates and formed 89 toxin gene profiles. The toxin genes sea, selk, selq and sell were significantly more common in MRSA, whilst tsst-1, seb, sed, selm, seln, selp and selj were more prevalent in MSSA (P < 0.001). The pvl gene was more commonly detected in CC59, whereas tsst-1 was more frequent in CC15, CC188 and ST398 (P < 0.001). The major genotypes were associated with specific antimicrobial resistance and toxin gene profiles. (C) 2013 Elsevier B. V. and the International Society of Chemotherapy. All rights reserved.