Histone H2B monoubiquitination regulates salt stress-induced microtubule depolymerization in Arabidopsis
Histone H2B monoubiquitination regulates salt stress-induced microtubule depolymerization in Arabidopsis
复制标题
组蛋白 H2B 单泛素化调节拟南芥中盐胁迫诱导的微管解聚
DOI：
10.1111/pce.12950
复制
发表时间：
2017-08-01
影响因子：
7.3
通讯作者：
Li, Yingzhang
中科院分区：
文献类型：
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作者：
Zhou, Sa;Chen, Qiuhong;Li, Yingzhang
组蛋白H_2B单素化(H_2Bub1)被认为是一种控制一系列细胞过程的调节机制。我们先前的研究表明，在拟南芥中，H_2Bub1参与了对生物胁迫的响应。然而，H_2Bub1在控制非生物胁迫反应中的分子调控机制仍然有限。在此，我们报道了组蛋白MONOUBIQUITINATION1(HUB1)和HUB2在盐胁迫响应中发挥了重要的调节作用。表型分析表明，H_2Bub1突变体对盐胁迫的耐受性降低。进一步分析表明，在盐胁迫下，H_2Bub1调控微管(MTS)解聚、蛋白磷酸酶(PTP1)和丝裂原活化蛋白激酶(MAP)磷酸酶(MKP)基因DsPTP1、mkp1、IBR5、PHS1的表达，并参与丝裂原活化蛋白Kase3(MAP kinase3、MPK3)和MPK6的激活。此外，酪氨酸磷酸化和MPK3和MPK6的激活都影响了MT在盐胁迫反应中的稳定性。因此，这些结果表明，H_2Bub1调节盐胁迫诱导的MT解聚，而PTP-MPK3/6信号模块负责整合调节MT稳定性的信号通路，这对植物耐盐性至关重要。
Histone H2B monoubiquitination (H2Bub1) is recognized as a regulatory mechanism that controls a range of cellular processes. We previously showed that H2Bub1 was involved in responses to biotic stress in Arabidopsis. However, the molecular regulatory mechanisms of H2Bub1 in controlling responses to abiotic stress remain limited. Here, we report that HISTONE MONOUBIQUITINATION1 (HUB1) and HUB2 played important regulatory roles in response to salt stress. Phenotypic analysis revealed that H2Bub1 mutants confer decreased tolerance to salt stress. Further analysis showed that H2Bub1 regulated the depolymerization of microtubules (MTs), the expression of PROTEINTYROSINE PHOSPHATASE1 (PTP1) and MAP KINASE PHOSPHATASE (MKP) genes - DsPTP1, MKP1, IBR5, PHS1, and was required for the activation of mitogen-activated protein kinase3 (MAP kinase3, MPK3) and MPK6 in response to salt stress. Moreover, both tyrosine phosphorylation and the activation of MPK3 and MPK6 affected MT stability in salt stress response. Thus, the results indicate that H2Bub1 regulates salt stress-induced MT depolymerization, and the PTP-MPK3/6 signalling module is responsible for integrating signalling pathways that regulate MT stability, which is critical for plant salt stress tolerance.