Identification of a novel coronavirus causing severe pneumonia in human: a descriptive study
Identification of a novel coronavirus causing severe pneumonia in human: a descriptive study
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DOI：
10.1097/cm9.0000000000000722
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发表时间：
2020-05-05
影响因子：
6.1
通讯作者：
Wang, Jian-Wei
中科院分区：
文献类型：
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作者：
Ren, Li-Li;Wang, Ye-Ming;Wang, Jian-Wei
背景人畜共患冠状病毒（CoV），包括严重急性呼吸综合征（SARS）-CoV和中东呼吸综合征（MERS）-CoV的人类感染，已引起全球范围内极大的公共卫生关注。在这里，我们报告了一种新的蝙蝠来源的冠状病毒引起严重和致命的肺炎在人类。方法收集武汉市金银潭医院收治的5例重症肺炎患者的临床资料和支气管肺泡灌洗（BAL）标本。提取BAL的核酸并进行下一代测序。进行病毒分离，并构建最大似然系统发育树。结果2019年12月18日至12月29日住院的5例患者均表现为发热、咳嗽、呼吸困难伴急性呼吸窘迫综合征并发症。胸片显示弥漫性阴影和实变。其中一名患者死亡。测序结果显示，在所有5名患者中存在一种以前未知的β-CoV毒株，分离株之间的核苷酸同源性为99.8%至99.9%。与SARS-CoV（GenBank NC_004718）的核苷酸同源性为79.0%，与MERS-CoV（GenBank NC_019843）的核苷酸同源性为51.8%。该病毒与蝙蝠SARS样冠状病毒（SL-ZC 45，GenBank MG 772933）在遗传学上最接近，具有87.6%至87.7%的核苷酸同一性，但处于单独的进化枝中。此外，这些病毒具有单个完整的开放阅读框基因8，作为蝙蝠来源的CoV的进一步指示。然而，试验性受体结合域的氨基酸序列与SARS-CoV相似，表明这些病毒可能使用相同的受体。结论发现了一种新的蝙蝠传播的CoV，与人类严重和致命的呼吸道疾病有关。
Background Human infections with zoonotic coronaviruses (CoVs), including severe acute respiratory syndrome (SARS)-CoV and Middle East respiratory syndrome (MERS)-CoV, have raised great public health concern globally. Here, we report a novel bat-origin CoV causing severe and fatal pneumonia in humans. Methods We collected clinical data and bronchoalveolar lavage (BAL) specimens from five patients with severe pneumonia from Wuhan Jinyintan Hospital, Hubei province, China. Nucleic acids of the BAL were extracted and subjected to next-generation sequencing. Virus isolation was carried out, and maximum-likelihood phylogenetic trees were constructed. Results Five patients hospitalized from December 18 to December 29, 2019 presented with fever, cough, and dyspnea accompanied by complications of acute respiratory distress syndrome. Chest radiography revealed diffuse opacities and consolidation. One of these patients died. Sequence results revealed the presence of a previously unknown beta-CoV strain in all five patients, with 99.8% to 99.9% nucleotide identities among the isolates. These isolates showed 79.0% nucleotide identity with the sequence of SARS-CoV (GenBank NC_004718) and 51.8% identity with the sequence of MERS-CoV (GenBank NC_019843). The virus is phylogenetically closest to a bat SARS-like CoV (SL-ZC45, GenBank MG772933) with 87.6% to 87.7% nucleotide identity, but is in a separate clade. Moreover, these viruses have a single intact open reading frame gene 8, as a further indicator of bat-origin CoVs. However, the amino acid sequence of the tentative receptor-binding domain resembles that of SARS-CoV, indicating that these viruses might use the same receptor. Conclusion A novel bat-borne CoV was identified that is associated with severe and fatal respiratory disease in humans.