Genome-Wide Analysis of Spatial Gene Expression in Arabidopsis Flowers
Genome-Wide Analysis of Spatial Gene Expression in Arabidopsis Flowers
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DOI：
10.1105/tpc.021741
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发表时间：
2004-05
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影响因子：
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通讯作者：
F. Wellmer;J. Riechmann;M. Alves-Ferreira;E. Meyerowitz
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文献类型：
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作者：
F. Wellmer;J. Riechmann;M. Alves-Ferreira;E. Meyerowitz
我们利用花特异性cDNA芯片和全基因组寡核苷酸芯片，比较了花同源突变体apetala1、apetala2、apetala3、雌蕊和agamous与野生型植物的花序基因表达谱。通过结合个体突变型/野生型比较的数据集，我们能够识别出大量的基因，这些基因在花中被预测为专门或至少主要在一种花器官中表达。我们通过原位杂交分析了其中几个基因的表达模式，发现它们与基于微阵列实验的预测相匹配。此外，我们的分析正确地分配了已知花器官特异性表达模式的基因。绝大多数已鉴定的转录本在雄蕊或心皮中发现，而少数基因被预测在萼片或花瓣中特异性或主要表达。这些结果表明，大量基因的空间受限表达是花发育的一部分，其程度在生殖器官和花被器官之间存在显著差异。
We have compared the gene expression profiles of inflorescences of the floral homeotic mutants apetala1, apetala2, apetala3, pistillata, and agamous with that of wild-type plants using a flower-specific cDNA microarray and a whole genome oligonucleotide array. By combining the data sets from the individual mutant/wild type comparisons, we were able to identify a large number of genes that are, within flowers, predicted to be specifically or at least predominantly expressed in one type of floral organ. We have analyzed the expression patterns of several of these genes by in situ hybridization and found that they match the predictions that were made based on the microarray experiments. Moreover, genes with known floral organ–specific expression patterns were correctly assigned by our analysis. The vast majority of the identified transcripts are found in stamens or carpels, whereas few genes are predicted to be expressed specifically or predominantly in sepals or petals. These findings indicate that spatially limited expression of a large number of genes is part of flower development and that its extent differs significantly between the reproductive organs and the organs of the perianth.