DNA METHYLTRANSFERASES - ACTIVITY MINIGEL ANALYSIS AND DETERMINATION WITH DNA COVALENTLY BOUND TO A SOLID MATRIX
DNA METHYLTRANSFERASES - ACTIVITY MINIGEL ANALYSIS AND DETERMINATION WITH DNA COVALENTLY BOUND TO A SOLID MATRIX
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DOI：
10.1016/0003-2697(85)90533-0
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发表时间：
1985-01-01
影响因子：
2.9
通讯作者：
SPADARI, S
中科院分区：
文献类型：
--
作者：
HUBSCHER, U;PEDRALINOY, G;SPADARI, S
我们描述了两种便于检测和表征DNA甲基转移酶的方法：活性凝胶分析和DNA-纤维素或DNA-琼脂糖在DNA甲基化反应中的应用。第一种方法允许确定催化亚基，确定蛋白质分解的影响，并进行进化或发育研究。第二种方法可以准确、快速地测定粗提物和提纯过程中的DNA甲基转移酶活性。
We describe two methods that facilitate detection and characterization of DNA methyltransferases: activity gel analysis and the use of DNA-cellulose or DNA-Sepharose in DNA methylation reactions. The first permits identification of catalytic subunits, determination of the influence of proteolysis, and evolutionary or developmental studies. The second allows accurate and fast determination of DNA methyltransferase activities in crude extracts and during purification.