Global genomic methylation levels in the liver and gonads of the three-spine stickleback (Gasterosteus aculeatus) after exposure to hexabromocyclododecane and 17-β oestradiol
Global genomic methylation levels in the liver and gonads of the three-spine stickleback (Gasterosteus aculeatus) after exposure to hexabromocyclododecane and 17-β oestradiol
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DOI：
10.1016/j.envint.2007.03.009
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发表时间：
2008-04-01
影响因子：
11.8
通讯作者：
Chipman, J. Kevin
中科院分区：
文献类型：
--
作者：
Aniagu, Stanley O.;Williams, Tim D.;Chipman, J. Kevin
DNA甲基化的改变是与非遗传毒性致癌物相关的主要表观遗传学机制。研究了六溴环十二烷(HBCD)、17-β雌二醇(E-2)和5-氮杂脱氧胞苷(5AdC)两种环境污染物对三刺鱼肝脏和性腺DNA甲基化水平(5-甲基2‘-脱氧胞苷)的影响。在30和300 ng/L的剂量下，六溴环己烷对雌性刺鱼肝脏的整体基因组甲基化没有显著影响。另一方面，甲基化抑制剂5-氮杂-2‘-脱氧胞苷显著降低了这些鱼肝脏的整体甲基化水平14%(P
Alteration of DNA methylation is a major epigenetic mechanism associated with the effects of nongenotoxic carcinogens. We evaluated the effects of two environmental pollutants, hexabromocyclododecane (HBCD), 17-beta oestradiol (E-2) as well as 5-aza 2' deoxycytidine (5AdC) on global DNA methylation levels (5-methyl 2' deoxycytidine) in the liver and gonads of the three-spine stickleback (Gasterosteus aculeatus). HBCD at 30 and 300 ng/L of water did not produce statistically significant differences in global genomic methylation in liver of female stickleback. On the other hand, the methylation inhibitor, 5-aza-2'-deoxycytidine, significantly lowered hepatic global methylation levels in these fish by 14% (P