History and genomic sequence analysis of the herpes simplex virus 1 KOS and KOS1.1 sub-strains
History and genomic sequence analysis of the herpes simplex virus 1 KOS and KOS1.1 sub-strains
复制标题
DOI：
10.1016/j.virol.2015.09.026
复制
发表时间：
2016-01-01
期刊：
影响因子：
3.7
通讯作者：
Knipe, David M.
中科院分区：
文献类型：
--
作者：
Colgrove, Robert C.;Liu, Xueqiao;Knipe, David M.
单纯疱疹病毒（HSV）株的基因组DNA序列的集合已经被定义和分析，并且一些关于病毒在培养物中有限传代后基因组稳定性的信息是可用的。广泛实验室传代后基因组变化的性质仍有待确定。在本报告中，我们回顾了HSV-1科斯实验室菌株和相关的KOS1.1实验室亚株（也称为科斯（M））的历史，并测定了科斯实验室亚株早期传代贮备液和KOS1.1亚株实验室贮备液的完整基因组序列。两个亚株的基因组高度相似，只有5个编码变化，20个非编码变化和大约20个非ORF序列变化。编码的变化可能解释了KOS1.1在高温下复制增加和神经侵袭性降低的表型特性。该研究还提供了序列标记，以确定特定实验室科斯病毒库存的来源。(C)2015 Elsevier Inc. All rights reserved.
A collection of genomic DNA sequences of herpes simplex virus (HSV) strains has been defined and analyzed, and some information is available about genomic stability upon limited passage of viruses in culture. The nature of genomic change upon extensive laboratory passage remains to be determined. In this report we review the history of the HSV-1 KOS laboratory strain and the related KOS1.1 laboratory sub-strain, also called KOS (M), and determine the complete genomic sequence of an early passage stock of the KOS laboratory sub-strain and a laboratory stock of the KOS1.1 sub-strain. The genomes of the two sub-strains are highly similar with only five coding changes, 20 non-coding changes, and about twenty non-ORF sequence changes. The coding changes could potentially explain the KOS1.1 phenotypic properties of increased replication at high temperature and reduced neuroinvasiveness. The study also provides sequence markers to define the provenance of specific laboratory KOS virus stocks. (C) 2015 Elsevier Inc. All rights reserved.