Evaluating the impact of the humic acid amendment on antibiotic resistance genes reduction and product quality during swine manure composting
Evaluating the impact of the humic acid amendment on antibiotic resistance genes reduction and product quality during swine manure composting
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DOI：
10.1016/j.jece.2023.110412
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发表时间：
2023-10
影响因子：
7.7
通讯作者：
Yousif Abdelrahman Yousif Abdellah-Yousif-Abdelrahman-Yousif-Abdellah-1701658828;Hong-Yu Chen;Shan-Shan Sun-Shan;Xi Yang;Yuan Luo;A. Bello;T. Mohamed;Rong Ren;Wan-Ting Li;Rashid Ahmed;Rui-Long Wang
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文献类型：
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作者：
Yousif Abdelrahman Yousif Abdellah-Yousif-Abdelrahman-Yousif-Abdellah-1701658828;Hong-Yu Chen;Shan-Shan Sun-Shan;Xi Yang;Yuan Luo;A. Bello;T. Mohamed;Rong Ren;Wan-Ting Li;Rashid Ahmed;Rui-Long Wang
抗生素在畜禽养殖场的过度使用及其随后在粪便中的积累导致堆肥产品中抗生素抗性基因（ARGs）的增加。然而，腐殖酸（HA）在堆肥过程中塑造抗生素和ARGs丰度，微生物结构和产品质量的功能仍不清楚。在这里，添加HA在减少抗生素和ARGs丰度在猪粪（SM）堆肥的效率进行了研究，使用5个率的HA：0%，1%，2%，4%和6%。结果表明，与对照堆肥相比，6%腐殖酸处理下，LSM、杆菌肽、大环内酯类、磺胺类、四环素类、万古霉素类ARGs和MacB、MexD、OleB、SrmB、Sul 1、VanHB、VanSB、pbp 2b、tetPB、tetX等ARGs亚型的丰度显著降低（P<0. 05）。此外，添加6%的HA形成了细菌群落（主要是不动杆菌属、假黄单胞菌属和黄单胞菌属）的丰度，以及碳水化合物代谢和氨基酸代谢水平（分别为6.9%和5.4%），这可以作为微生物活性的指标，从而加速抗生素的减少。RDA分析表明，TOC、水分含量和pH值对微生物活性和ARGs去除率有显著影响（P< 0.05）。堆肥质量评价结果表明，添加6% HA显著提高了堆肥中腐殖物质和HA的总含量，分别为120.7 ± 1.02 g/kg和62.3 ± 0.92 g/kg，降低了富里酸的含量，为12.8 ± 1.22 g/kg，从而促进了抗生素和ARGs的减少。总的来说，这项研究提供了一个很有前途的选择，提高堆肥的质量和安全性，减轻ARGs在自然环境中的循环。
The overuse of antibiotics in livestock farms and their subsequent accumulation in manures lead to increase antibiotic resistance genes (ARGs) in the compost products. Yet, the function of humic acid (HA) in shaping antibiotics and ARGs abundances, microbial structure, and product quality during composting remains unclear. Here, the efficiency of added HA in reducing antibiotics and ARGs abundances during swine manure (SM) composting was studied using five rates of HA: 0%, 1%, 2%, 4%, and 6%. The findings indicated that the abundance of ARGs types such as LSM, bacitracin, macrolide, sulfonamide, tetracycline, and vancomycin and ARGs subtypes includingMacB,MexD,OleB,SrmB,Sul1,VanHB,VanSB,pbp2b,tetPB, andtetXdecreased significantly under 6% HA amount compared with CK compost (P< 0.05). Besides, the added 6% HA shaped the abundance of the bacterial community (notablyAcinetobacter,Pseudoxanthomonas, andLuteimonas), as well as the level of carbohydrate metabolism and amino acid metabolism (6.9% and 5.4%, respectively), which can be taken as an indicator for microbial activity, thereby accelerating antibiotics decrease. RDA analysis revealed that TOC, moisture content, and pH considerably influenced microbial activity and ARGs reduction (P< 0.05). The estimation of compost quality indicated that 6% HA significantly improved the total content of humic substance and HA to 120.7 ± 1.02 g/kg and 62.3 ± 0.92 g/kg, and reduced the content of fulvic acid to 12.8 ± 1.22 g/kg, thus promoting antibiotics and ARGs reduction. Overall, this study offers a promising option for improving compost quality and safety and mitigating ARGs circulation in the natural environment.