ChrASO, the chromate efflux pump of Shewanella oneidensis, improves chromate survival and reduction.

ChrASO, the chromate efflux pump of Shewanella oneidensis, improves chromate survival and reduction.
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DOI:
10.1371/journal.pone.0188516
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发表时间:
2017
期刊:
影响因子:
3.7
通讯作者:
Fons M
Fons M
中科院分区:
综合性期刊3区
文献类型:
--
作者:
Baaziz H;Gambari C;Boyeldieu A;Ali Chaouche A;Alatou R;Méjean V;Jourlin-Castelli C;Fons M

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将编码基因chrASO的铬酸盐外排泵基因定位于锥状希瓦氏杆菌MR1的染色体上。虽然chrASO在没有铬酸盐的情况下表达,但它的表达水平随着铬(VI)的加入而增加。当被删除时,所产生的突变体Δchraso与野生型菌株相比表现出对铬酸盐敏感的表型。有趣的是,chrASO在大肠杆菌中的异源表达使其对高浓度的铬酸盐具有抵抗力。此外,chrASO在金黄色葡萄球菌和大肠杆菌中的表达显著促进了铬(VI)的还原。这种效应可能是胞质外铬酸盐还原的结果,也可能是由于更好的细胞存活率导致了更强的铬(VI)还原。
The chromate efflux pump encoding gene chrASO was identified on the chromosome of Shewanella oneidensis MR1. Although chrASO is expressed without chromate, its expression level increases when Cr(VI) is added. When deleted, the resulting mutant ΔchrASO exhibits a chromate sensitive phenotype compared to that of the wild-type strain. Interestingly, heterologous expression of chrASO in E. coli confers resistance to high chromate concentration. Moreover, expression of chrASO in S. oneidensis and E. coli significantly improves Cr(VI) reduction. This effect could result either from extracytoplasmic chromate reduction or from a better cell survival leading to enhanced Cr(VI) reduction.
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