ChrASO, the chromate efflux pump of Shewanella oneidensis, improves chromate survival and reduction.
ChrASO, the chromate efflux pump of Shewanella oneidensis, improves chromate survival and reduction.
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DOI:
10.1371/journal.pone.0188516
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发表时间:
2017
期刊:
影响因子:
3.7
通讯作者:
Fons M
中科院分区:
文献类型:
--
作者:
Baaziz H;Gambari C;Boyeldieu A;Ali Chaouche A;Alatou R;Méjean V;Jourlin-Castelli C;Fons M
The chromate efflux pump encoding gene chrASO was identified on the chromosome of Shewanella oneidensis MR1. Although chrASO is expressed without chromate, its expression level increases when Cr(VI) is added. When deleted, the resulting mutant ΔchrASO exhibits a chromate sensitive phenotype compared to that of the wild-type strain. Interestingly, heterologous expression of chrASO in E. coli confers resistance to high chromate concentration. Moreover, expression of chrASO in S. oneidensis and E. coli significantly improves Cr(VI) reduction. This effect could result either from extracytoplasmic chromate reduction or from a better cell survival leading to enhanced Cr(VI) reduction.
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