Polymorphisms of IGFI contribute to the development of ischemic stroke
Polymorphisms of IGFI contribute to the development of ischemic stroke
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DOI：
10.3892/etm.2011.372
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发表时间：
2012-01-01
影响因子：
2.7
通讯作者：
Kim, Young Ock
中科院分区：
文献类型：
--
作者：
Kim, Hak Jae;Kim, Su Kang;Kim, Young Ock
胰岛素样生长因子1（IFG 1）在局灶性脑缺血动物模型中具有神经保护作用，并与老年人缺血性卒中（IS）结局相关。在这项研究中，我们调查是否IFG 1基因的单核苷酸多态性（SNPs）与发展和IS的临床特征在韩国人口。共招募了119名IS患者和289名对照受试者。根据美国国立卫生研究院卒中调查（NIHSS; = 6）和改良Barthel指数（MBI; = 60）的评分将卒中患者分为亚组。在IFG 1基因的SNPs中，选择5个SNPs并通过直接测序进行分析：rs 2162679（内含子）、rs 2195239（内含子）、rs 978458（内含子）、rs 1520220（内含子）和rs6214（3'非翻译区; 3' UTR）。采用多元logistic回归模型分析遗传数据。使用SNPStats、SNPAnalyzer Pro和Helixtree程序计算比值比（OR）、95%置信区间（CI）和p值。两个SNPs，rs 2162679和rs6214，与IS的发展相关。经Bonferroni校正（pc）后，IS组rs 2162679和rs6214的A、G等位基因频率与对照组比较差异有统计学意义[rs 2162679，OR（95%CI）= 1.64（1.17-2.31），p=0.004，p（c）=0.02; rs6214，OR（95%CI）= 1.52（1.12-2.07），p=0.007，p（c）=0.035]。然而，选定的五个SNP与NIHSS和MBI评分无关。这些结果表明，IGF 1可能与IS的发展。
Insulin-like growth factor 1 (IFG1) is neuroprotective in animal models of focal brain ischemia and correlates with ischemic stroke (IS) outcome in the elderly. In this study, we investigated whether single nucleotide polymorphisms (SNPs) of the IFG1 gene are associated with the development and clinical features of IS in a Korean population. A total of 119 patients with IS and 289 control subjects were recruited. Stroke patients were classified into subgroups according to the scores of the National Institutes of Health Stroke Survey (NIHSS; = 6) and the Modified Barthel Index (MBI; = 60). Among the SNPs of the IFG1 gene, five SNPs were selected and analyzed by direct sequencing: rs2162679 (intron), rs2195239 (intron), rs978458 (intron), rs1520220 (intron) and rs6214 (3' untranslated region; 3'UTR). Multiple logistic regression models were conducted to analyze genetic data. SNPStats, SNPAnalyzer Pro and Helixtree programs were used to calculate odds ratios (ORs), 95% confidence intervals (CIs) and p-values. Two SNPs, rs2162679 and rs6214, were associated with the development of IS. After Bonferroni correction (pc), the A and G alleles of rs2162679 and rs6214 had significant differences between patients with IS and the controls [rs2162679, OR (95% Cl) = 1.64 (1.17-2.31), p=0.004, p(c)=0.02; rs6214, OR (95% CI) = 1.52 (1.12-2.07), p=0.007, p(c)=0.035], respectively. However, the five selected SNPs were not related to the NIHSS and MBI scores. These results suggest that IGF1 may be associated with the development of IS.