Characterization of individual polynucleotide molecules using a membrane channel
Characterization of individual polynucleotide molecules using a membrane channel
复制标题
DOI：
10.1073/pnas.93.24.13770
复制
发表时间：
1996-11-26
影响因子：
11.1
通讯作者：
Deamer, DW
中科院分区：
文献类型：
--
作者：
Kasianowicz, JJ;Brandin, E;Deamer, DW
我们证明了电场可以驱动单链RNA和DNA分子通过脂质双层膜中直径2.6 nm的离子通道。由于通道直径只能容纳单链RNA或DNA，因此每种聚合物都以延伸链的形式穿过膜，部分阻断通道。每个分子的通过被检测为离子电流的短暂减少，其持续时间与聚合物长度成正比，通道阻塞因此可以用来测量多核苷酸的长度。随着进一步的改进，该方法原则上可以提供DNA或RNA单分子碱基序列的直接、高速检测。
We show that an electric field can drive single-stranded RNA and DNA molecules through a 2.6-nm diameter ion channel in a lipid bilayer membrane. Because the channel diameter can accommodate only a single strand of RNA or DNA, each polymer traverses the membrane as an extended chain that partially blocks the channel. The passage of each molecule is detected as a transient decrease of ionic current whose duration is proportional to polymer length, Channel blockades can therefore be used to measure polynucleotide length. With further improvements, the method could in principle provide direct, high-speed detection of the sequence of bases in single molecules of DNA or RNA.