Regulation of Hormonal Control, Cell Reprogramming, and Patterning during De Novo Root Organogenesis
Regulation of Hormonal Control, Cell Reprogramming, and Patterning during De Novo Root Organogenesis
复制标题
DOI：
10.1104/pp.17.00980
复制
发表时间：
2018-02-01
期刊：
影响因子：
7.4
通讯作者：
Manuel Perez-Perez, Jose
中科院分区：
文献类型：
--
作者：
Bustillo-Avendano, Estefano;Ibanez, Sergio;Manuel Perez-Perez, Jose
通过形成新器官的身体再生是发育生物学中的一个主要问题。我们研究了从头根形成拟南芥（拟南芥）的整个叶片。我们的研究结果表明，当地的细胞分裂素的生物合成和生长素的生物合成在叶片生长素长距离运输到叶柄导致增殖的J 0121标记的木质部相关的组织和其他通过信号转导的吲哚-3-乙酸诱导28（IAA 28），起重机（IAA 18），木腿，和拟南芥响应调节因子1（ARR 1），ARR 10，和ARR 12。脉管系统增殖还涉及细胞周期调节因子KIP相关蛋白2和异常侧根形成4，导致大量具有类似愈伤组织形成的生根能力的细胞。内源性愈伤组织的形成先于胚后根建立者细胞的特化，根通过短根、PLETHORA 1（PLT 1）和PLT 2的活性从胚后根建立者细胞开始。shr plt 1 plt 2突变体的原基起始被阻断。干细胞调节剂SCHIZORIZA、JACKELAND、BLUEJAY和SCARECROW也参与根的发生，并且需要形成新的器官，因为突变体显示出混乱和减少的层数和组织起始，导致生根减少。我们的工作提供了一个器官再生模型，通过从头根形成，说明关键阶段和主要途径。
Body regeneration through formation of new organs is a major question in developmental biology. We investigated de novo root formation using whole leaves of Arabidopsis (Arabidopsis thaliana). Our results show that local cytokinin biosynthesis and auxin biosynthesis in the leaf blade followed by auxin long-distance transport to the petiole leads to proliferation of J0121-marked xylem-associated tissues and others through signaling of INDOLE-3-ACETIC ACID INDUCIBLE28 (IAA28), CRANE (IAA18), WOODEN LEG, and ARABIDOPSIS RESPONSE REGULATORS1 (ARR1), ARR10, and ARR12. Vasculature proliferation also involves the cell cycle regulator KIP-RELATED PROTEIN2 and ABERRANT LATERAL ROOT FORMATION4, resulting in a mass of cells with rooting competence that resembles callus formation. Endogenous callus formation precedes specification of postembryonic root founder cells, from which roots are initiated through the activity of SHORT-ROOT, PLETHORA1 (PLT1), and PLT2. Primordia initiation is blocked in shr plt1 plt2 mutant. Stem cell regulators SCHIZORIZA, JACKDAW, BLUEJAY, and SCARECROW also participate in root initiation and are required to pattern the new organ, as mutants show disorganized and reduced number of layers and tissue initials resulting in reduced rooting. Our work provides an organ regeneration model through de novo root formation, stating key stages and the primary pathways involved.