Pot1, the putative telomere end-binding protein in fission yeast and humans
Pot1, the putative telomere end-binding protein in fission yeast and humans
复制标题
DOI：
10.1126/science.1060036
复制
发表时间：
2001-05-11
期刊：
影响因子：
56.9
通讯作者：
Cech, TR
中科院分区：
文献类型：
--
作者：
Baumann, P;Cech, TR
纤毛原生动物的端粒蛋白与大核染色体末端富含C的单链DNA结合。我们现在已经在裂变酵母和人类中鉴定出同源蛋白质。这些Pot1（端粒保护）蛋白各自结合其自身端粒重复序列的富含G的链，与保护染色体末端的直接作用一致。酵母分裂pot 1（+）基因的缺失对染色体稳定性有直接影响，导致端粒DNA的快速丢失和染色体环化。现在看来，覆盖染色体末端的蛋白质广泛分布在真核生物界。
Telomere proteins from ciliated protozoa bind to the single-stranded C-rich DNA extensions at the ends of macronuclear chromosomes. We have now identified homologous proteins in fission yeast and in humans. These Pot1 (protection of telomeres) proteins each bind the G-rich strand of their own telomeric repeat sequence, consistent with a direct role in protecting chromosome ends. Deletion of the fission yeast pot1(+) gene has an immediate effect on chromosome stability, causing rapid loss of telomeric DNA and chromosome circularization. It now appears that the protein that caps the ends of chromosomes is widely dispersed throughout the eukaryotic kingdom.