Probiotic Bifidobacterium bifidum G9-1 ameliorates phytohemagglutinin-induced diarrhea caused by intestinal dysbiosis
Probiotic Bifidobacterium bifidum G9-1 ameliorates phytohemagglutinin-induced diarrhea caused by intestinal dysbiosis
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DOI：
10.1111/1348-0421.12743
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发表时间：
2019-10-09
影响因子：
2.6
通讯作者：
Yamamura, Hideki
中科院分区：
文献类型：
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作者：
Makizaki, Yutaka;Maeda, Ayako;Yamamura, Hideki
腹泻主要是由伴随大肠杆菌过度增殖的生态失调引起的。coli）。虽然目前的治疗方法可以解决症状，但它们不能抑制肠道中致病菌的增殖。益生菌对宿主健康有许多有益的影响，包括恢复肠道微生物群的平衡。本研究调查了益生菌两歧双歧杆菌G9-1（BBG 9 -1），这是积极的肠道生态失调，腹泻的发病率，肠道菌群的组成，并在植物血凝素（PHA）诱导的腹泻大鼠模型的肠道组织的影响。用PHA处理大鼠，有和没有BBG 9 -1，并使用高通量16 S rRNA测序检查整个肠道和粪便的微生物群组成。与以前的报道一致，PHA给药引起腹泻以及由于E.大肠杆菌过度增殖。组织学观察表明，空肠绒毛长度缩短。接受BBG 9 -1的大鼠在生态失调、腹泻症状和空肠绒毛长度方面表现出明显的改善。主坐标分析表明，BBG 9 -1组和正常组之间的微生物群谱比PHA组和正常组之间的微生物群谱更相似。这些结果表明BBG 9 -1抑制E.大肠杆菌和恢复空肠绒毛长度，从而改善生态失调，并反过来，减轻腹泻症状。
Diarrhea is largely caused by dysbiosis accompanying the hyperproliferation of Escherichia coli (E. coli). While current treatments can resolve the symptoms, they cannot suppress the proliferation of pathogenic bacteria in the intestine. Probiotics have numerous beneficial effects on host health, including restoring the balance of the intestinal microbiota. This study investigated the effect of the probiotic Bifidobacterium bifidum G9-1 (BBG9-1), which is active in intestinal dysbiosis, in the incidence of diarrhea, in the composition of the intestinal microbiota, and in the intestinal tissue of a rat model of phytohemagglutinin (PHA)-induced diarrhea. The rats were treated with PHA, with and without BBG9-1, and the microbiota composition throughout the intestine and stool was examined using high-throughput 16S rRNA sequencing. In line with previous reports, PHA administration caused diarrhea as well as dysbiosis due to E. coli hyperproliferation. Histological findings indicated that the jejunal villus length was shortened. Rats that received BBG9-1 showed clear improvements in dysbiosis, diarrhea symptoms, and jejunal villus length. Principal coordinates analysis demonstrated the microbiota profile to be more similar between the BBG9-1 and normal groups than between the PHA and normal groups. These results indicated that BBG9-1 suppresses the hyperproliferation of E. coli and restores the jejunal villus length, thereby improving dysbiosis, and in turn, alleviating the symptoms of diarrhea.